丁香实验_LOGO
登录
提问
我要登录
|免费注册
点赞
收藏
wx-share
分享

COMBINED ALCIAN BLUE - P.A.S

互联网

3297

 

SECTIONS
4micron paraffin wax sections
 

SOLUTIONS

1. Alcian Blue pH 2.5

 

  • Alcian Blue 1g
  • 3% Acetic Acid 100ml

2. 1% Periodic Acid

3. Schiffs Reagent
 

METHOD

 

  1. Take sections to water
  2. Place in Alcian Blue for 5 minutes
  3. Wash in distilled water
  4. Place in 1% periodic acid for 10 minutes
  5. Rinse well in distilled water
  6. Place in Schiffs reagent for 20 minutes
  7. Wash in running tap water for 10 minutes
  8. Stain lightly in Gills Haematoxylin
  9. Wash and 'blue' in 1% Lithium carbonate
  10. Dehydrate, clear . Mount sections in DPX

RESULTS

 

  • Acid Mucins - Blue
  • Neutral Mucins - Magenta
  • Nuclei - Pale Blue

 

提问
扫一扫
丁香实验小程序二维码
实验小助手
丁香实验公众号二维码
扫码领资料
反馈
TOP
打开小程序