COMBINED ALCIAN BLUE - P.A.S
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3302
SECTIONS
4micron paraffin wax sections
SOLUTIONS
1. Alcian Blue pH 2.5
- Alcian Blue 1g
- 3% Acetic Acid 100ml
2. 1% Periodic Acid
3. Schiffs Reagent
METHOD
- Take sections to water
- Place in Alcian Blue for 5 minutes
- Wash in distilled water
- Place in 1% periodic acid for 10 minutes
- Rinse well in distilled water
- Place in Schiffs reagent for 20 minutes
- Wash in running tap water for 10 minutes
- Stain lightly in Gills Haematoxylin
- Wash and 'blue' in 1% Lithium carbonate
- Dehydrate, clear . Mount sections in DPX
RESULTS
- Acid Mucins - Blue
- Neutral Mucins - Magenta
- Nuclei - Pale Blue