PRINS Combined With Indirect Immunofluorescence

Primed in situ labeling (PRINS) has proven to be an attractive alternative to fluorescence in situ hybridization for in situ DNA labeling and for being combined on the same slide with others methods. For the aim of a study on the asymmetrical segregation of the chromosomes of the megakaryocytes during polyploidization, we developed a simultaneous PRINS and immunofluorescent labeling. We report here the method, which enabled us to visualize the centromere of selected chromosomes and the achromatic spindle on the same picture, a method that may be of a more general application.