Nylon cDNA Expression Arrays

Nucleic acid arrays provide a powerful methodology for studying biological systems on a genomic scale. BD Atlas™ Arrays, developed by BD Biosciences Clontech, are expression profiling products specifically designed to be accessible to all laboratories performing isotopic blot hybridization experiments. We have developed two types of readily accessible BD Atlas Arrays: nylon macroarrays, well suited for high-sensitivity expression profiling using a limited gene set, and broad-coverage plastic microarrays, for a more extensive analysis of a comprehensive set of genes. In this chapter, we describe protocols for printing and performing gene expression analysis using nylon membrane-based arrays. For a more in-depth description and protocols related to plastic film-based arrays, please refer to Chapter 3 .