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Modulation of Calpain-Mediated Protein Kinase C Activation Within Intact Cells

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An inherent consequence of calpain activation in situ is activation of the ubiquitous signal transduction kinase, protein kinase C (PKC). Whether or not one is directly interested in the effects of PKC, it may be important during experimental design and interpretation to consider that certain PKC-mediated events can essentially compete with calpain-mediated proteolysis. This chapter provides some general approaches that we have developed in our ongoing studies for modulating calpain-mediated PKC activation.
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