丁香实验_LOGO
登录
提问
我要登录
|免费注册
点赞
收藏
wx-share
分享

Differentiation of Endothelial Cells from Human Embryonic Stem Cells and Induced Pluripotent Stem Cells

互联网

495
Endothelial cells line the entire circulatory system and form the interface between the blood vessel intima and the circulating red blood cells. Endothelial cells are crucial to the proper function of the circulatory system and tissue viability, including their roles in coagulation, fibrinolysis, inflammation, and most specifically, vasculogenesis and angiogenesis. Given the importance of endothelial cells in vascular formation, it is essential to expand our knowledge of endothelial cell physiology and growth. The vasculogenic and angiogenic properties that enable new vascular networks to form and consequently perfuse ischemic tissues make endothelial cells an essential element of potential novel therapies. In this chapter, we describe a three-step technique to derive endothelial cells from human embryonic stem cells or induced pluripotent stem cells using a three-dimensional embryoid body formation protocol. A technique to derive a highly pure endothelial population using flow cytometry will also be discussed.
提问
扫一扫
丁香实验小程序二维码
实验小助手
丁香实验公众号二维码
扫码领资料
反馈
TOP
打开小程序