Acrylamide Urea Gel (35 ml)

 

Acrylamide Urea Gel (35 ml)

 

	10%	15%
40/2% acrylamide	10 ml	13.1 ml
10X TBE	3.5 ml	3.5 ml
Urea	15 g	15 g
H20	10ml	7.0 ml

 

Microwave ~10 seconds and stir until dissolved

Degas 1-2 min

Add 0.3 ml 10% ammonium persulfate

Add 35 microliters TEMED.

Pour Gel and wait at least 20 minutes before removing comb.

The gel can be stored overnight at room temp. if the top of the gel is wet with water and is wrapped tightly in Saran Wrap