核酸标记技术检测法

核酸标记技术检测法

　　通过核酸标记技术可将细胞因子cDNA作为基因探钊检测细胞内细胞因子 基因组 DNA或mRNA。主要有以下几种方法：

1.应用同位素（或非同位素）标记的cDNA探针，检测经Northern blot后细胞因子mRNA水平或采用打点杂交法。

2.应用标记cDNA探钊与细胞或组织切片进行原位杂交，然后进行放射自显影。

3.细胞因子mRNA经反转录为cDNA，用特异性细胞因子引物经聚合酶链反应（PCR）扩增细胞因子cDNA,Southern blot后用标记探针检测特异细胞因子DNA水平。

（金伯泉）

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