SSC-Molecular Biology
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Hi,Can you tell me what do "SSC" in a southern blot, how he act on DNA or in the protocole.
(excuse-me for my traduction, i never write in english)
-Sebela-
"SSC" is a abbreviation of sodium chloride and sodium citrate solution, which used to transfer nuclear acid from gel to membrane ( 20 *SSC or 1undefinedSSC or undefinedSSC with alkaline). In Roche's DIG system, they said that the high solt concentration SSC shows much efficiently than the others like valcuum or electrophoresis.
-zhongmindai-
DNA is transferred most efficiently to nitrocellulose as a sodium salt where 90% of the effective negative charge has been neutralized by sodium chloride/citrate. Good Luck.
-phdconsult-
