Analysis of HSV Polypeptides

Lytic infection by herpes simplex virus (HSV) efficiently inhibits the synthesis of most cellular proteins while a large number of viral proteins is produced, including a host shut off protein (1 –3 ). The inhibitory effect of the protein obviously needs a certain period of time to be fully efficient, but some cellular proteins involved in virus replication still are produced. Examples are those interacting with the regulatory viral proteins V_MW 65 (65K_TIF ) and IE 110 (V_MW 110, ICPO) (4 –8 ). Certain quiescent cellular genes are activated after infection to express their proteins (9 ).