Flow Cytometry Analysis of an In Situ PCR for the Detection of Human Immunodeficiency Virus Type-1 (HIV-1) Proviral DNA
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The polymerase chain reaction (PCR) (1 ,2 ) is extremely sensitive and flexible, and in theory, will detect a single copy of a specific DNA (or retrotranscribed RNA) sequence either in cell cultures or in clinical samples (3 ). PCR technology has, therefore, been applied to the diagnosis of a wide range of clinical conditions, in particular, infectious diseases (4 –6
