Fastfilter Plasmid Midi Kit Spin Protocol
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实验步骤
Lyse bacterial cells with Alkaline-SDS Solution:
6. Add 2 ml Buffer GBT, cover and gently mix by inverting tube 3-4 times.
Clear the lysate with Lysate Clearance Filter Syringe:
Purify Plasmid DNA with HiBindTM DNA Midi Column:
Elution Plasmid DNA From HiBindTM DNA Midi column: