Molecular Weight Marker

Molecular Weight Marker

1. Digest 20 µg l DNA (40 µl DNA if at 0.5 µg/µl)
2. Add 50 µl 10X Tracking Dye
3. Bring volume of the digested DNA up to 500 µl with H2O (410 µl if the DNA is 40 µl): If l DNA is digested in our lab, the volume of H2O varys.
4. Mix, heat to 65 C for 5 min, cool quickly on ice, and store at 4 C.
5. Load 5 µl (200 ng per application)

l Digest Marker DNA Size (in base pair)s and Quantity (5 µl application per lane)

Pst
I Bst
II Hind
III
 11,497 8,454 23,130 95 ng DNA
  5,077 7,242  9,416 38.8
  4,749 6,369  6,557 27
  4,507 5,686  4,507 18.5
  2,828 4,822  2,322  9.6
  2,559 4,324  2,027  8.4
  2,443 2,323    125
  2,140 1,929
  1,986 1,371
  1,700 1,264
  1,159   702
  1,093   224
    795   224
    514 
    468
    448
    339
    274
    247
    216
    211
    200
    164
    150
     94
     87
     75
     72