TaqMan PCR以及ABI-7700的简单介绍
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ABI PRISM® 7700 Sequence Detection System
Integrates a PCR-based assay with laser scanning technology to excite fluorescent dyes present in the specially designed TaqMan® probes. It is a fully integrated system for real-time detection of PCR. The system includes a built-in thermal cycler, a laser to induce fluorescence, CCD (charge-coupled device) detector, real-time sequence detection software, and TaqMan® reagents for the fluorogenic 5' nuclease assay. The cycle-by-cycle detection of the increase in the amount of PCR product is quantified in real time as the special probes, "reporter dye", fluoresces when the "quencher" is removed from the fragment during the PCR extension cycle.
Application of the "TaqMan® Probe" to Gene expression problems
To induce fluorescence during PCR, laser light is distributed to the 96 sample wells via a multiplexed array of optical fibers. The resulting fluorescent emission returns via the fibers and is directed to a spectrograph with a charge-coupled device (CCD) camera.
Sophisticated precision optics provides highly reproducible and quantitative results
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