绿色荧光蛋白的应用

1 、骨架和细胞分裂

 

Kevin Sullivan's 实验室
酵母菌内 SPB 和微管动力学
酵母菌中肌动蛋白的动力
果蝇中 MEI-S332 蛋白
果蝇有丝分裂和 mRNA 运输
网丙菌属细胞骨架
RNA 剪切因子的核内运输
网丙菌属的趋化作用
网丙菌属中细胞骨架动力和细胞运动
核动力
网丙菌属中细胞动力
细胞骨架动力和胞内运输

 

 

 

2、细胞器动力学和泡囊运输

 

用 GFP 显示小囊运输
用 GFP 观察 TGN 运输
细胞骨架动力学和胞内运输

 

 

 

3 、 发育生物学

 

用 GFP 观察线虫的神经发育
分析果蝇神经发育的不对称性细胞分裂
线虫 Lin-14
Fischbach lab
用 GFP 观察网丙菌属的形态发生学
GFP 在小鼠发育中的标记方法

 

 

 

4 、 神经生物学

 

神经极性发育
tracking intracellular transport of peptide neurotransmitters using GFP Allen Lab
Enhancer trapping using tau-GFP as reporter localized to axons

 

 

 

5、其他应用

 

Cell surface organization in Natural Killer cells Dan Davis
localization of calmodulin in S. pombe with GFP
GFP-plakoglobin and expression plasmids Klymkowsky lab
Molecular Motion Laboratory Yale University
Measuring diacylglycerol in vivo with a GFP-PKC chimera Tobias Meyer
Bentley Lab using GFP for on-line bioprocess monitoring & control
Tracking viral proteins with GFP fusions

 

 

 

6、GFP vectors and technology

 

Clontech Inc. Supplier of constructs for making GFP fusions
DeltaVision 3D microscopy platform optimized for imaging GFP in living cells in real time
GFP Expression Truly Amazing Web Site about GFP technology
Universal Imaging Corp. makers of the MetaGFP image processing platform
Quantum Biotechnologies Inc Suppliers of GFP and BFP constructs
GFP in Arabidopsis
BabCo/Covance antibody to GFP
Lightools GFP plate illumination systems
GFP in Chlamydomonas

 

 

 

7 、 Other Interesting GFP Links

 

 

GFP Resources for Teachers
Picture of Aequoria victoria, source of GFP
Structure of green fluorescent protein Full text of Yang et al Nature Structural Bio paper
Table of GFP mutant forms
Optics in Cell Biology