酵母转化

 

・         Yeast Transformation (Gietz Lab)
LiAc/SS-DNA/PEG Transformation

・         Yeast Transformation (Breeden Lab)
LiAc method

・         Large-Scale Yeast Transformation (Yale Yeast Analysis Center)
Detailed protocol for large-scale yeast transformation including competent cell preparation, transfromation...

・         Yeast Transformation ( Herskowitz Lab)

・         Sleazy Plate Transformation of Yeast ( Herskowitz Lab)

・         Transformation of Yeast - LiAc Method (Lazo Lab)  

・         Two Hybrid System Transformation (Gietz Lab)
This  Protocol is for 2 Hybrid system screens

・         Yeast Transformation (Fangman/Brewer lab)
Yeast transformation protocol  Using Frozen Competent Cells and Single-stranded DNA as a Carrier

・         Quick Yeast Transformation (Gietz Lab)
This Protocol allows for transformation with any Yeast cell source. When just a few transformants are sufficient, such as the transformation of a test plasmid or a GAL4BD fusion plasmid, the following protocol can be used. This procedure is very flexible and can be applied to yeast cells from a number of different sources; however, for best results, use cells from a freshly grown plate.

・         Yeast Transformation (D. Amberg)
Electroporation method

・         Fission Yeast Transformation (Forsburg Lab) 
Includes three methods for transforming S. pombe with recombinant DNA: electroporation, lithium acetate treatment, and protoplast fusion. 

・         Multiwell Transformation  (Davis Lab)
Detailed protocol with LiAcetate PEG transformation method

・         Transformation of Yeast (PMCI Research)

・         "Lazy Bones" Plasmid Transformation (Dohlman Lab) 
A quick method for plasmid transformation into yeast.

・         Competent Yeast Preparation (Gietz Lab)
This Protocol allows you to prepare frozen yeast cells that are competent for transformation after thawing

・         Yeast Transformation (Gottschling Lab)
Provides LiAc method, "lazy bones" method and electroporation method 

・         Yeast Transformation (Finley Lab) 
LiOAc method

• Yeast Transformation with DMSO/LioAc (high efficiency) (Hahn Lab)
    
• Yeast Transformation (Rapid method) (Hahn Lab)
  LiOAc method

・         Plasmid Rescue from Yeast from Liquid Culture and Clony Rescue (Gottschling Lab)

・         Yeast Colony PCR (Gottschling Lab)
For yeast transformant screening

・         Direct PCR from Whole Yeast Cells (Namjin Chung)
Zymolyase Method for screening yeast transformants