丁香实验_LOGO
登录
提问
我要登录
|免费注册
点赞
收藏
wx-share
分享

Footprinting with Exonuclease III

互联网

672
Within the last few years footprinting techniques have become increasingly important in the study of protein-nucleic acid interactions. This is partly the result of a fast-growing number of known nucleic acid binding proteins but also because of an increase in the available probes that can be chosen in order to tackle a specific problem. There are two major groups of probes-the chemical probes and the enzymatic probes. These enzymatic probes, such as DNase I or exonuclease III, have the advantage of acting specifically at the DNA. Chemical probes are often less specific and react also with the protein. This can disturb the correct interaction of protein and DNA. For the study of very fragile protein-DNA complexes, therefore, enzymatic probes are often preferable.
提问
扫一扫
丁香实验小程序二维码
实验小助手
丁香实验公众号二维码
扫码领资料
反馈
TOP
打开小程序