PCR基本实验方法（二）
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Recommended Reaction Conditions:
Initial Conditions:
Initial denaturation at start: 92 - 97oC for 3 - 5 min. If you denature at 97oC, denature sample only; add rest of mix after reaction colls to annealing temperature (prevents premature denaturation of enzyme).
Initial annealing temperature: as high as feasible for 3 min (eg: 50 - 75oC). Stringent initial conditions mean less non-specific product, especially when amplifying from eukaryotic genomic DNA.
Initial elongation temperature: 72oC for 3 - 5 min. This allows complete elongation of product on rare templates.
