Balb/c 3T3 Cytotoxicity Test
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The cytotoxic effect of chemicals on Balb/c 3T3 cells in culture is measured by cell viability (Neutral Red Uptake) and total cell protein (Kenacid Blue R dye binding method).
Healthy Balb/c 3T3 cells (an established cell line), when maintained in culture, continuously divide and multiply over time. The basis of this test is that a cytotoxic chemical (regardless of site or mechanism of action) will interfere with this process and, thus, result in a reduction of the growth rate as reflected by cell number. The degree of inhibition of growth, related to the concentration of the test compound, provides an indication of toxicity.
