Separation of Epidermal Tissue from Underlying Dermis and Primary Keratinocyte Culture

The epidermis shares many structural similarities to other epithelia throughout the body. All epithelia function as a barrier protecting the internal organs. The epidermis of the skin protects the exterior of the body, whereas other forms of epithelia line the airways, blood vessels, and gastrointestinal, urinary, and reproductive tracts. Some glandular epithelia secret substances such as sweat, mucus, and hormones. All epithelia are avascular and consist of closely packed cells, which are tightly attached to one another via cell junctions. This tight structure allows all epithelia to closely regulate the movement of materials such as ions, nutrients, and secretory products (1 ,2 ).