Immunoblot Analysis and Band Depletion Assays

Western blotting has been widely utilized to detect various polypeptides or polypeptide epitopes (e.g., posttranslational modifications) within cells (reviewed in 1 -4 ). If the signals in samples being analyzed are compared to a suitable standard curve and appropriate internal standards are utilized to confirm equivalent loading of various samples, Western blotting appears to be a suitable method of quantitating polypeptides as well.