The Use of Double-Stranded RNA to Knock Down Specific Gene Activity

In many eukaryotes, the introduction of double-stranded RNA (dsRNA) into cells triggers the degradation of cognate mRNAs through a posttranscriptional gene silencing mechanism. This phenomenon has been called RNA interference or RNAi. Several methods for delivering dsRNA into the model organism C. elegans are described; these methods include (1) microinjecting dsRNA synthesized in vitro into the body cavity of the worm, (2) soaking worms in a solution of dsRNA, (3) feeding worms dsRNA-expressing bacteria, and (4) engineering transgenic worm strains to express dsRNA in vivo. Variations of these methods may be used to perform RNAi in other species as well. The choice of which delivery method to use, along with other options (region to target, length of dsRNA) are also discussed.