Cryopreservation of Transgenic Rat Lines
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The following protocol is for freezing rat blastocysts by direct plunging into liquid nitrogen (1 ). Although this protocol is designed for freezing the embryos in polypropylene cryotubes, it could readily be adapted to 0.5-mL straws. For a general discussion of cryopreservation, the reader is referred to Chapter 25.
