CD45/CD45RA/CD45RB/CD45RC/CD45RO

互联网2010-12-02

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CD45/CD45RA/CD45RB/CD45RC/CD45RO

CD45

常用单克隆抗体或代号： T29/33,BMAC1;(T200,B220)

主要表达细胞： Leu [NL]

分子质量(kDa)和结构： gp180- 240,白细胞共同抗原(LCA)

功能： PTP酶,调节信号传导

CD45RA

常用单克隆抗体或代号： G1- 15,F8- 11- 13,Leu18,2H4;(限制性LCA)

主要表达细胞： Tsub,B,G,M [NL]

分子质量(kDa)和结构： gp205- 220(含A外显子编码产物异型)

功能：调节信号传导

CD45RB

常用单克隆抗体或代号： PT17/26/16;(限制性LCA)

主要表达细胞： Tsub,B,M,Mac,G [NL]

分子质量(kDa)和结构： gp205- 220(含B外显子编码产物异型)

功能：调节信号传导

CD45RC

常用单克隆抗体或代号： (限制性LCA)

主要表达细胞： T,B [NL]

分子质量(kDa)和结构： gp200- 220(含C外显子编码产物的异型 )

功能：调节信号传导

CD45RO

常用单克隆抗体或代号： UCHL1;(限制性LCA)

主要表达细胞： Thy,Tsub,Bsub,(G,M)[NL]

分子质量(kDa)和结构： gp180(无A、B和C外显子编码产物异型)

功能：与CD22结合,调节信号传导

CD45

Aka leukocyte common antigen (LCA); a tyrosine phosphatase

Critical requirement for T and B cell antigen receptor-mediated activation

Target of immunosuppressive antibody treatment

Major component of glycocalyx

Negative in patients with severe combined immunodeficiency, characterized by a defect in function or B and T cell development, lymphopenia, and deficiency in humoral and cell-mediated immunity.

Different subsets of hemaoietic cells express different isoforms, which can change in response to cytokines:

CD45RA - naive/resting T cells, medullary thymocytes

CD45RO - memory/activated T cells, cortical thymocytes

Positive staining (normal): all hemaoietic cells, stronger in lymphocytes (10% of surface area)

Negative staining: red blood cells, non-hemaoietic cells, lymphoplasmacytic lymphoma, lymphoblastic lymphoma, anaplastic lymphoma, multiple myeloma, Reed-Sternberg cells

Micro images: Reed-Sternberg cells-CD45 negative, figure 3C

CD45RO

Aka UCHL-1

Positive staining (normal): memory and activated T cells, some B cells, granulocytes (weak), macrophages (weak), cortical thymocytes

Positive staining (disease): 75% of T cell non-Hodgkin’s lymphomas, variable T cell ALL/LBL, 25% of AML

Negative staining: Most B cell non-Hodgkin’s lymphomas, carcinomas

Micro images: nasal NK/T cell lymphoma (figure C)