求救：看一下引物设计的如何？急！

我的基因的mRNA序列如下：
1 atgaatggtg acacgcgggc cgcagtggtg acctcaccac ctccaaccac agcccctcac
61 aaggagaggt actttgacag agtcgatgag aacaatccag aatatttgcg ggagaggaac
121 atggcaccag accttcgtca ggacttcaat atgatggagc agaagaagag ggtgtctatg
181 attctgcaga gtcccgcctt ctgtgaagag ctggagtcta tgatacagga gcagtttaag
241 aaggggaaga accctacagg cctgttggca ttacagcaga ttgcggattt catgacggcg
301 agtgtgccca atgtctaccc ggcagctcca caaggaggaa tggccgccct gaacatgagt
361 cttggtatgg ttactcctgt gaatgacctt agaggatctg attctattgc atatgacaag
421 ggggagaagt tactgcgatg taagctggca gcattttaca gactagcaga cctctttggc
481 tggtctcagc ttatctacaa tcacatcaca accagagtga actctgagca ggagcacttc
541 ctcatcgtgc ctttcggact tctctacagt gaagtgactg cgtccagcct ggttaaggtc
601 aatctacaag gagacatagt agaccgcgga agtactaacc tgggagtcaa ccaggccggc
661 ttcaccttgc actcggccat ttatgctgca cgaccagatg ccaagtgcat tgtgcacatt
721 cacacaccag caggggctgc ggtctctgca atgaagtgtg ggctcttgcc tatttccccg
781 gaggcgcttt cccttggaga ggttgcttat catgactatc atggcattct ggttgatgaa
841 gaggaaaaaa ttttgattca gaagaatctg ggccctaaaa gcaaggttct cattctccgg
901 aatcacgggc tcgtgtcagt tggagagagt gtggaggagg ccttctttta tatccacaac
961 cttgtggttg catgtgagat ccaggttcga actctggcaa gtgctggagg accagacaac
1021 ttagttctgc tggatcctgg gaagtacaaa gccaaatccc gttctccagg gactccagca
1081 ggggaaggct ctggatcacc tcccaagtgg cagattgggg agcaggaatt tgaagctctt
1141 atgcggatgc tcgataatct gggctacaga actggctacc cctatcgata ccctgctctg
1201 agagagagat ctaaaaagta cagcgatgtg gaagtccctg ccagtgtcac cggccactcc
1261 tttgctagtg acggcgattc gggcacttgc tcgccgctca gacacagttt tcagaagcag
1321 cagcgagaga agacaagatg gctgaactct ggccggggtg atgacgcttc tgaggagggg
1381 cagaacggaa gcagtcccaa gtcgaagact aagtggacta aagaggatgg acatagaact
1441 tccacctctg ctgtccctaa cctgtttgtt ccattgaaca ctaacccaaa agaggtccag
1501 gagatgagga acaagatccg agagcagaac ttacaggaca ttaagacggc aggacctcag
1561 tcgcaggttt tgtgtggtgt gatgatggac agaagccttg tccaggggga gctcgtgacg
1621 gcctccaaag ccatcattga gaaggaatac cagcctcatg taattgtgag caccacaggt
1681 cccaacccct tcaacacgct cacagaccgt gaactagagg aataccgccg agaggtggag
1741 cggaagcaga agggctctga agagaacctg gacgagacaa gagagcagaa agagaagagt
1801 cctccagacc agtcagctgt tcccaacact ccgcccagca ctcccgtcaa gcttgaagga
1861 ggccttcccc aggagccgac atccagggat ggcagcggat gccacgcctc caagccgact
1921 ccccctgacc tctcccctga tgagccttca gaagcacttg ccttcccaac agtggcagag
1981 gaagcccatg cttccccaga caccactcag ccccttgctg aggctgaccc tgagccagcc
2041 tcagcctcag ctccaggagc tgaagaggta gcctcccctg ccactgagga ggggtctccc
2101 atggaccctg gcagcgatgg gtctccaggc aagtccccat ccaagaagaa gaagaagttc
2161 cgcaccccgt ccttcctgaa gaagagcaag aagaagagtg actcctga
//
在外文参考文献上的引物为：
forward primer,5-CAGGCCGGCTTCACCTT-3;
reverse primer, 5-GCACAATGCACTTGGCATCT-3;
请大家帮帮忙，看一下该引物如何？扩增的片段多大？谢谢了！急！