SELDI Technology for Identification of Protein Biomarkers

The ProteinChip SELDI System and chip arrays provide a sensitive, high-throughput methodology for protein biomarker discovery. The combination of retentate chromatography and time-of-flight mass spectrometry allows for the detection of increased peak numbers even when processing microliter sample volumes. The multiple chip chemistries allow the flexibility for user-defined protocols that can facilitate protein purification strategies. In addition, ProteinChip arrays can be designed to characterize antibody-antigen and other protein-protein interactions. This review will update the reader on the current SELDI methods and protocols that are especially pertinent to protein biomarker discovery.