Immunofluorescence
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<center> <b><font>Immunofluorescence</font> </b></center>
<center> <b><font>of </font> <i><font>C. elegans</font> </i> <font> Embryos</font> </b></center>
Materials:
|
fixative |
dependent on antigen (common: 4% formaldehyde or 100% MeOH) |
|
post-fixative |
dependent on antigen (usually MeOH) |
|
PBS |
1X PBS |
|
PBST |
PBS + detergent (0.05 to 0.5 % Tween or Triton) |
|
DAPI (1000X) |
1 ug/ml in EtOH |
|
Antifade (BIORAD) |
|
|
Block |
1X PBS 1% BSA 10% Donkey Serum 0.02% Azide |
