Blotting知识介绍

The transfer of biomolecules onto a membrane support

1)Preparing a cell-free extract containing the biomolecule(s) of interest
2)Resolve the mixture by gel electrophoresis
3)Transfer the gel onto a membrane support
4)Incubate the membrane with a detection system that specifically binds to the molecule of interest

Southerns - Detection of DNA fragments on a blot
Northerns - Detection of RNA fragments on a blot
Westerns - Detection of proteins on a blot
South-Westerns - Detection of DNA bound to proteins on a blot