DNA fragmentation analysis protocol
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实验步骤
2. Lyse cells in 0.5 ml detergent buffer: 10 mm Tris (pH 7.4), 5 mm EDTA, 0.2% Triton
4. Incubate on ice for 30 min.
5. Centrifuge at 27000 g for 30 minutes
6. Divide supernatants into 2-250 μl aliquots
7. Add 50 ul ice cold 5 M NaCl to each followed by vortexing
9. Add 2 μl (10 mg/ml) DNase free RNase. Incubate for 5 hr at 37o C.
11. Extract DNA with phenol, choloform, isoamyl alcohol and precipitate with EtOH.
