Long Term Storage of Transformed E.coli
互联网
Long Term Storage of Transformed E.coli
- Transfer 10 ml of 250 ml overnight culture in sterile flip-cap 15 ml Tube.
- Add sterile Glycerol to 15 % final conc. (i.e. 1.875 ml of 80% Glycerine stock solution).
- Store at -80°C.