What is the highest temperature that reverse trascriptases can be used
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Question
What is the highest temperature that SUPERSCRIPT II, MMLV, or THERMOSCRIPT can be used?
Answer
For RT-PCR, SUPERSCRIPT II RT can be used up to 50 C. MMLV can be used at temperatures up to 42 C. THERMOSCRIPT can be used at temperatures as high as 70 C (especially for amplicons expected to be 1 kb or less). For PCR products expected to be greater than 1 kb a maximum first strand synthesis temperature of 65oC is suggested. Be sure your first-strand primer anneals at the high temperature.
