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Differential Methylation Hybridization Using CpG Island Arrays

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Differential hybridization and its related techniques have been developed to identify genes whose expressions are altered during different physiological conditions or in dissimilar cell or tissue types (1 3 ). High-throughput DNA array technologies have further advanced these analyses, providing for simultaneous examinations of expressions of thousands of genes between two different cell types in a single experiment (4 6 ). Recently, we have adapted the hybridization approach to devise an array-based technique, called differential methylation hybridization (DMH), to identify changes in DNA methylation patterns commonly observed in cancer (7 ). Methylation of DNA is the addition of a methyl group to the 5th carbon position of cytosine that is 5′ to a guanine in GC-rich regions known as CpG islands, which are frequently located in the 5′-end of regulatory regions of genes (8 ,9 ) This epigenetic reaction does not usually change nucleotide sequences nor affect the specificity of DNA base pairing, but it can have inhibitory effects on gene expression (8 ,9 ).
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