DNA Purification from Gels
互联网
627
1.Remove gel slice contain DNA fragment and place in 10 volumes of:
300 mM NaOAc,pH 7.0 300 ml 1 M NaOAC,pH 7.0
1 mM EDTA 2 ml 500 mM EDTA,pH 8.0
698 ml ddH20
2.Incubate at 22℃ for 30 min.Transfer gel slice to a fresh tube.
3.Place tube in a Dry Ice/Ethanol bath for 5 min.
4.Puncture the bottom of a 0.5 ml microcentrifuge tube with a needle.Place the gel slice into this tube.Place this tube inside a 1.5 ml microcentrifuge tube.
5.Centrifuge for 15 min.
6.Collect the Eluent from the 1.5 ml eppendorf tube.Extract and precipitate the DNA.
