Attachment of Reporter and Conjugate Groups to the 3′ Termini of Oligonucleotides
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- Abstract
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- Literature Cited
Abstract
Conjugation of oligonucleotides at the 3 terminus is less common because this site is used for covalent linkage to solid?phase oligonucleotide synthesis supports. However, 3?oligonucleotide conjugates have several valuable physicochemical properties, including their ability to stabilize nucleic acid hybridization complexes and to retard the activity of exonucleases. This unit discusses methods for preparing oligonucleotides conjugated at the 3 terminus.
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- Modified Solid Phase Synthesis Supports and Phosphoramidites
- Solid Phase Synthesis Supports for Producing 3′‐Fuctional Groups Suitable for Conjugation
- Postsynthetic Conjugation
- Summary
- Literature Cited
- Figures
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Figure 4.5.1 Generic oligonucleotide 3′‐conjugate. View Image -
Figure 4.5.2 Solid phase supports for attaching polyethylene glycol (S.2 ) and cholesterol (S.3 ) to the 3′ termini of oligonucleotides. View Image -
Figure 4.5.3 Solid support for conjugating biotin (S.4 ) or other conjugants (S.5 ) to oligonucleotides at their 3′ termini. View Image -
Figure 4.5.4 A universal support for preparing 3′‐modified oligonucleotides. View Image -
Figure 4.5.5 Thioester support for the preparation of 3′‐modified oligonucleotides. View Image -
Figure 4.5.6 Supports for the release of oligonucleotides containing 3′‐thiols (S.8 ) and 3′‐aldehydes (S.9 ). View Image -
Figure 4.5.7 Supports for the release of oligonucleotides containing various 3′‐functional groups. View Image -
Figure 4.5.8 Photolabile and Pd(0)‐labile orthogonal oligonucleotide synthesis supports. View Image -
Figure 4.5.9 Introduction of dyes and porphyrins at the 3′ termini of oligonucleotides. View Image -
Figure 4.5.10 Postsynthetic modification of oligonucleotide 3′‐carboxylic acids. View Image -
Figure 4.5.11 Postsynthetic modification of oligonucleotide 3′‐thiols. View Image -
Figure 4.5.12 Postsynthetic modification of oligonucleotide 3′‐phosphorothioates. View Image -
Figure 4.5.13 Template‐mediated oligonucleotide coupling. View Image -
Figure 4.5.14 Postsynthetic modification of oligonucleotides by reductive amination. View Image -
Figure 4.5.15 Solution phase conjugation of protected oligonucleotides. View Image -
Figure 4.5.16 Oligonucleotide conjugates and bis‐conjugates prepared from protected oligonucleotides. View Image -
Figure 4.5.17 Oligonucleotide conjugates prepared from protected substrates containing 3′‐alkyl‐amines. View Image -
Figure 4.5.18 Topological control of nucleopeptide formation. View Image -
Figure 4.5.19 Solution phase synthesis of nucleopeptides. View Image -
Figure 4.5.20 Support for the linear synthesis of nucleopeptides. View Image -
Figure 4.5.21 Modification of surfaces by conjugation of 3′‐derivatized oligonucleotides. View Image
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Literature Cited
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