CE Analysis of -Globulin Mobility and Potential Clinical Utility
互联网
442
Serum protein electrophoresis is widely used in clinical laboratories to measure the relative abundance of each obtained fraction.
Moreover, we found that the migration time of the γ-globulin fraction can be reproducibly determined (CV = 1.1%). Immunoglobulins
were purified from serum using protein l
-agarose and their N
-glycosylation was studied using CE on a DNA sequencer. Liver fibrosis patients showed a lower level of sialylation and this
moderately correlates with the migration time of the γ-globulins (r
= 0.2–0.4). This allowed us to differentiate healthy individuals from these patients with an acceptable diagnostic accuracy
(area under the curve = 0.75). This glycomics approach could become a significant added value to a daily, routine clinical
test.