FFPE Direct PCR Kit
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797
实验步骤
1. Protocol for FFPE Extraction
3) Incubate at 60°C for 30-60 minutes.
4) Incubate at 90°C for 60 minutes.
5) Add 100 μl FP3 Buffer and vortex to mix.
6) Store the extraction at 2-8°C.
1) Thaw primer solutions. Keep on ice after complete thawing, and mix well before use.
3) Prepare one of the following reaction mixes on ice: (For a 25 μl reaction volume)
4) Gently mix the reaction and spin down in microcentrifuge.
5) Set up program for a routine PCR reactions:
Initial Denaturation 94-95°C for 1-5 min
25-40 cycles 94-95°C for 30 sec
