Agarose Gel Electrophoresis for the Separation of DNA Fragments
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实验材料
实验步骤
2. Setting up of Gel Apparatus and Separation of DNA Fragments
2) Program the power supply to desired voltage (1-5V/cm between electrodes).
7) Turn on the power. Run the gel until the dye has migrated to an appropriate distance.
3. Observing Separated DNA fragments
1) When electrophoresis has completed, turn off the power supply and remove the lid of the gel box.
4) Properly dispose of the gel and running buffer per institution regulations.
1. Sambrook, J. & Russell, D.W. Molecular Cloning, 3rd edition (2001).
5. Aaji, C. & Borst, P. The gel electrophoresis of DNA. Biochim. Biophys. Acta. 269, 192-200 (1972).