Overview of Biacore Systems and Their Applications
互联网
2970
- Abstract
- Table of Contents
- Literature Cited
Abstract
Surface plasmon resonance (SPR) allows for the investigation of the functional nature of binding interactions and provides detailed kinetic information across a wide range of molecular weights, including small molecules, all without the use of labels. Here the various Biacore instrument platforms and their primary uses, ranging from semi?automated systems designed for simple, flexible basic research to fully automated, high?throughput systems, and systems designed to function in regulated environments, are all highlighted. The available sensor chip surfaces and immobilization techniques are also discussed. Biacore SPR biosensors can be used for a wide variety of assays, including specificity, active concentration measurement, kinetics, and affinity and thermodynamic parameters. Biacore SPR biosensors, which measure real?time analysis of biospecific interactions without the use of labeled molecules, can be used for a wide variety of protein interaction assays. In this unit, examples and recommendations for studying protein interactions with a variety of molecules are provided. This unit also shows how the technology can be used to determine binding specificity, active concentration measurements, and the determination of kinetic and thermodynamic parameters.
Keywords: Biacore; SPR; surface plasmon resonance; biosensor; label?free; protein interaction analysis
GO TO THE FULL PROTOCOL:
PDF or HTML at Wiley Online LibraryTable of Contents
- Biacore System Overview
- Surface Preparation
- Assay Types
- Protein Interaction Analysis
- Literature Cited
GO TO THE FULL PROTOCOL:
PDF or HTML at Wiley Online LibraryMaterials
GO TO THE FULL PROTOCOL:
PDF or HTML at Wiley Online LibraryFigures
Videos
Literature Cited
| Abad, L.W., Neumann, M., Tobias, L., Obenauer‐Kutner, L., Jacobs, S., and Cullen, C. 2002. Development of a biosensor‐based method for detection and isotyping of antibody responses to adenoviral‐based gene therapy vectors. Anal. Biochem. 310:107‐113. | |
| Aggarwal, S., Janssen, S., Wadkins, R.M., Harden, J.L., and Denmeade, S.R. 2005. A combinatorial approach to the selective capture of circulating malignant epithelial cells by peptide ligands. Biomaterials 26:6077‐6086. | |
| Alaedini, A. and Latov, N. 2001. A surface plasmon resonance biosensor assay for measurement of anti‐GM(1) antibodies in neuropathy. Neurology 56:855‐860. | |
| Backman, D. and Danielson, U.H. 2003. Kinetic and mechanistic analysis of the association and dissociation of inhibitors interacting with secreted aspartic acid proteases 1 and 2 from Candida albicans. Biochim. Biophys. Acta 1646:184‐195. | |
| Baerga‐Ortiz, A., Bergqvist, S., Mandell, J.G., and Komives, E.A. 2004. Two different proteins that compete for binding to thrombin have opposite kinetic and thermodynamic profiles. Protein Sci. 13:166‐176. | |
| Boehm, H., Boehringer, M., Bur, D., Gmuender, H., Huber, W., Klaus, W., Kostrewa, D., Kuehne, H., Luebbers, T., Meunier‐Keller, N., and Mueller, F. 2000. Novel inhibitors of DNA gyrase: 3D structure based biased needle screening, hit validation by biophysical methods, and 3D guided optimization. A promising alternative to random screening. J. Med. Chem. 43:2664‐2674. | |
| Bokken, G.C., Corbee, R.J., van Knapen, F., and Bergwerff, A.A. 2003. Immunochemical detection of Salmonella group B, D and E using an optical surface plasmon resonance biosensor. FEMS Microbiol. Lett. 222:75‐82. | |
| Borch, J. and Roepstorff, P. 2004. Screening for enzyme inhibitors by surface plasmon resonance combined with mass spectrometry. Anal. Chem. 76:5243‐5248. | |
| Casper, D., Bukhtiyarova, M., and Springman, E.B. 2004. A Biacore biosensor method for detailed kinetic binding analysis of small molecule inhibitors of p38alpha mitogen‐activated protein kinase. Anal. Biochem. 325:126‐136. | |
| Clark, W.A., Jian, X., Chen, L., and Northup, J.K. 2001. Independent and synergistic interaction of retinal G‐protein subunits with bovine rhodopsin measured by surface plasmon resonance. Biochem J. 358:389‐397. | |
| Day, Y.S., Baird, C.L., Rich, R.L., and Myszka, D.G. 2002. Direct comparison of binding equilibrium, thermodynamic, and rate constants determined by surface‐ and solution‐based biophysical methods. Protein Sci. 11:1017‐1025. | |
| Day, Y.S.N. and Myszka, D. 2003. Characterizing a drug's binding site on albumin. J. Pharm. Sci. 92:333‐343. | |
| de Haro, L., Ferracci, G., Opi, S., Iborra, C., Quetglas, S., Miquelis, R., Leveque, C., and Seagar, M. 2004. Ca2+/ calmodulin transfers the membrane‐proximal lipid‐binding domain of the v‐SNARE synaptobrevin from cis to trans bilayers. Proc. Natl. Acad. Sci. U.S.A. 101:1578‐1583. | |
| Deinum, J., Gustavsson, L., Gyzander, E., Kullman‐Magnusson, M., Edstrom, A., and Karlsson, R. 2002. A thermodynamic characterization of the binding of thrombin inhibitors to human thrombin, combining biosensor technology, stopped‐flow spectrophotometry, and microcalorimetry. Anal. Biochem. 300:152‐162. | |
| Dhalluin, C., Ross, A., Huber, W., Gerber, P., Brugger, D., Gsell, B., and Senn, H. 2005. Structural, kinetic, and thermodynamic analysis of the binding of the 40 kDa PEG‐interferon‐alpha2a and its individual positional isomers to the extracellular domain of the receptor IFNAR2. Bioconjugate Chem. 16:518‐527. | |
| Dubs, M.‐C., Altschuh, D., and Van Regenmortel, M.H.V. 1991. Interaction between viruses and monoclonal antibodies studied by surface plasmon resonance. Immunol. Lett. 31:59‐64. | |
| Fägerstam, L.G., Frostell, Å., Karlsson, R., Kullman, M., Larsson, A., Malmqvist, M., and Butt, H. 1990. Detection of antigen‐antibody interactions by surface plasmon resonance. Application to epitope mapping. J. Mol. Recognit. 3:208‐214. | |
| Fisher, R.D., Wang, B., Alam, S.L., Higginson, D.S., Robinson, H., Sundquist, W.I., and Hill, C.P. 2003. Structure and ubiquitin binding of the ubiquitin‐interacting motif. J. Biol. Chem. 278:28976‐28984. | |
| Frostell‐Karlsson, A., Remaeus, A., Roos, H., Anderson, K., Borg, P., Hämäläinen, M., and Karlsson, R. 2000. Biosensor analysis of the interaction between immobilized human serum albumin and drug compounds for prediction of human serum albumin binding levels. J. Med. Chem. 43:1986‐1992. | |
| Gilligan, J.J., Schuck, P., and Yergey, A.L. 2002. Mass spectrometry after capture and small‐volume elution of analyte from a surface plasmon resonance biosensor. Anal. Chem. 74:2041‐2047. | |
| Gossas, T. and Daielson, U.H. 2003. Analysis of the pH‐dependencies of the association and dissociation kinetics of HIV‐1 protease inhibitors. J. Mol. Recognit. 16:203‐212. | |
| Huber W. 2005 A new strategy for improved secondary screening and lead optimization using high‐resolution SPR characterization of compound‐target interactions. J. Mol. Recognit. 18:273‐281. | |
| Jokiranta, T.S., Hellwage, J., Koistinen, V., Zipfel, P.F., and Meri, S. 2000. Each of the three binding sites on complement factor H interacts with a distinct site on C3b. J. Biol. Chem. 275:27657‐27662. | |
| Karlsson, R. 1999. Affinity analysis of non‐steady‐state data obtained under mass transport limited conditions using Biacore technology. J. Mol. Recognit. 12:285‐292. | |
| Karlsson, R. and Fält, A. 1997. Experimental design for kinetic analysis of protein protein interactions with surface plasmon resonance biosensors. J. Immunol. Methods 200:121‐133. | |
| Karlsson, R., Kullman‐Magnusson, M., Hämäläinen, M.D., Remaeus, A., Andersson, K., Borg, P., Gyzander, E., and Deinum, J. 2000. Biosensor analysis of drug‐target interactions: Direct and competitive binding assays for investigation of interactions between thrombin and thrombin inhibitors. Anal. Biochem. 278:1‐13. | |
| Karlsson, R. and Larsson A. 2004. Affinity measurement using surface plasmon resonance. Methods Mol. Biol. 248:389‐415. | |
| Karlsson, O.P. and Löfås, S. 2002. Flow‐mediated on‐surface reconstitution of G‐protein coupled receptors for applications in surface plasmon resonance biosensors. Anal. Biochem. 300:132‐138. | |
| Katsamba, P.S., Park, S., and Laird‐Offringa, I.A. 2002. Kinetic studies of RNA‐protein interactions using surface plasmon resonance. Methods 26:95‐104. | |
| Kikuchi, J., Furukawa, Y., and Hayashi, N. 2003. Identification of novel p53‐binding proteins by biomolecular interaction analysis combined with tandem mass spectrometry. Mol. Biotechnol. 23:203‐212. | |
| Lieberg, B., Nylander, C., and Lundström, I. 1983. Surface plasmon resonance for gas detection and biosensing. Sensors Actuators 4:299‐304. | |
| Leonard, P., Hearty, S., Wyatt, G., Quinn, J., and O'Kennedy, R. 2005. Development of a surface plasmon resonance‐based immunoassay for Listeria monocytogenes. J. Food Prot. 68:728‐735. | |
| Lopez, F., Pichereaux, C., Burlet‐Schiltz, O., Pradayrol, L., Monsarrat, B., and Esteve, J.P. 2003. Improved sensitivity of biomolecular interaction analysis mass spectrometry for the identification of interacting molecules. Proteomics 3:402‐412. | |
| Maesawa, C., Inaba, T., Sato, H., Iijima, S., Ishida, K., Terashima, M., Sato, R., Suzuki, M., Yashima, A., Ogasawara, S., Oikawa, H., Sato, N., Saito, K., and Masuda, T. 2003. A rapid biosensor chip assay for measuring of telomerase activity using surface plasmon resonance. Nucl. Acids Res. 31:E4‐4. | |
| McFarland, B.J. and Strong, R.K. 2003. Thermodynamic analysis of degenerate recognition by the NKG2D immunoreceptor: Not induced fit but rigid adaptation. Immunity 19:803‐812. | |
| Medina, M.B. 2004. Binding interaction studies of the immobilized Salmonella typhimurium with extracellular matrix and muscle proteins, and polysaccharides. Int. J. Food Microbiol. 93:63‐72. | |
| Myszka, D.G. 1999. Improving biosensor analysis. J. Mol. Recognit. 12:1‐6. | |
| Myszka, D.G. 2000. Kinetic, equilibrium, and thermodynamic analysis of macromolecular interactions with Biacore. Methods Enzymol. 323:325‐340. | |
| Myszka, D.G., He, X., Dembo, M., and Morton, T.A. 1998. Extending the range of rate constants available from Biacore: Interpreting mass transport‐influenced binding data. Biophys. J. 75:583‐594. | |
| Natsume, T., Taoka, M., Manki, H., Kume, S., Isobe, T., and Mikoshiba, K. 2002. Rapid analysis of protein interactions: On‐chip micropurification of recombinant protein expressed in Esherichia coli. Proteomics 2:1247‐1253. | |
| Navratilova, I., Sodroski, J., and Myszka, D.G. 2005. Solubilization, stabilization, and purification of chemokine receptors using biosensor technology. Anal. Biochem. 339:71‐81. | |
| Nelson, R.W., Nedelkov, D., and Tubbs, K.A. 2000. Biomolecular interaction analysis mass spectometry. BIA/MS can detect and characterize protiens in complex biological fluids at the low‐ to subfemtomole level. Anal. Chem. 72:404A‐411A. | |
| Nordin, H., Jungnelius, M., Karlsson, R., and Karlsson, O.P. 2005. Kinetic studies of small molecule interactions with protein kinases using biosensor technology. Anal. Biochem. 340:359‐368. | |
| Peter, J.C., Briand, J.P., and Hoebeke, J. 2003. How biotinylation can interfere with recognition: A surface plasmon resonance study of peptide‐antibody interactions. J. Immunol. Methods 274:149‐158. | |
| Quinn, J.G., O'Kennedy, R., Smyth, M., Moulds, J., and Frame, T. 1997. Detection of blood group antigens utilising immobilised antibodies and surface plasmon resonance. J. Immunol. Methods 206:87‐96. | |
| Rich, R.L., Hoth, L.R., Geoghegan, K.F., Brown, T.A., LeMotte, P.K., Simons, S.P., Hensley, P., and Myszka, D.G. 2002. Kinetic analysis of estrogen receptor/ligand interactions. Proc. Natl. Acad. Sci. U.S.A. 99:8562‐8567. | |
| Rich, R.L. and Myszka, D.G. 2001. Survey of the year 2000 commercial optical biosensor literature. J. Mol. Recognit. 14:273‐294. | |
| Rini, B., Wadhwa, M., Bird, C., Small, E., Gaines‐Das, R., and Thorpe, R. 2005. Kinetics of development and characteristics of antibodies induced in cancer patients against yeast expressed rDNA derived granulocyte macrophage colony stimulating factor (GM‐CSF). Cytokine 29:56‐66. | |
| Roos, H., Karlsson, R., Nilshans, H., and Persson, A. 1998. Thermodynamic analysis of protein interactions with biosensor technology. J. Mol. Recognit. 11:204‐210. | |
| Schier, R. and Marks, J.D. 1996. Efficient in vitro affinity maturation of phage antibodies using Biacore guided selections. Hum. Antibody Hybridomas 7:97‐105. | |
| Schlattner, U., Dolder, M., Wallimann, T., and Tokarska‐Schlattner, M. 2001. Mitochondrial creatine kinase and mitochondrial outer membrane porin show a direct interaction that is modulated by calcium. J. Biol. Chem. 276:48027‐48030. | |
| Schuster, S.C., Swanson, R.V., Alex, L.A., Bourret, R.B., and Simon, M.I. 1993. Assembly and function of a quaternary signal transduction complex monitored by surface plasmon resonance. Nature 365:343‐346. | |
| Seet, B.T., McCaughan, C.A., Handel, T.M., Mercer, A., Brunetti, C., McFadden, G., and Fleming, S.B. 2003. Analysis of an orf virus chemokine‐binding protein: Shifting ligand specificities among a family of poxvirus viroceptors. Proc. Natl. Acad. Sci. U.S.A 100:15137‐15142. | |
| Shuman, F.S., Hämäläinen, M.D., and Danielson, U.H. 2004. Kinetic and thermodynamic characterization of HIV‐1 protease inhibitors. J. Mol. Recognit. 17:106‐119. | |
| Stenlund, P., Babcock, G.J., Sodroski, J., and Myszka, D.G. 2003. Capture and reconstitution of G protein–coupled receptors on a biosensor surface. Anal. Biochem. 316:243‐250. | |
| Swanson, S.J. 2003. New technologies for the detection of antibodies to therapeutic proteins. Dev Biol (Basel). 112:127‐133. | |
| Thai, C.T. and Ogata, R.T. 2005. Recombinant C345C and factor I modules of complement components C5 and C7 inhibit C7 incorporation into the complement membrane attack complex. J. Immunol. 174:6227‐6232. | |
| Thorpe, R. and Swanson, S.J. 2005. Assays for detecting and diagnosing antibody‐mediated pure red cell aplasia (PRCA): An assessment of available procedures. Nephrol. Dial. Transplant. 20(Suppl 4):iv16‐22. | |
| Tseng, M.C. and Chu, Y.H. 2005. Using surface plasmon resonance to directly identify molecules in a tripeptide library that bind tightly to a vancomycin chip. Anal. Biochem. 336:172‐177. | |
| Tsoi, P.Y. and Yang, M. 2002. Kinetic study of various binding modes between human DNA polymerase beta and different DNA substrates by surface‐plasmon‐resonance biosensor. Biochem. J. 361:317‐325. | |
| Van Regenmortel, M.H. 2003. Improving the quality of Biacore‐based affinity measurements. Dev Biol (Basel) 112:141‐151. | |
| Yoshitani, N., Satou, K., Saito, K., Suzuki, S., Hatanaka, H., Seki, M., Shinozaki, K., Hirota, H., and Yokoyama, S. 2005. A structure‐based strategy for discovery of small ligands binding to functionally unknown proteins: Combination of in silico screening and surface plasmon resonance measurements. Proteomics 5:1472‐1480. | |
| Young, P.J., Day, P.M., Zhou, J., Androphy, E.J., Morris, G.E., and Lorson, C.L. 2002. A direct interaction between the survival motor neuron protein and p53 and its relationship to spinal muscular atrophy. J. Biol. Chem. 277:2852‐2859. | |
| Zeder‐Lutz, G., Zuber, E., Witz, J., and Van Regenmortel, M.H.V. 1997. Thermodynamic analysis of antigen‐antibody binding using biosensor measurements at different temperatures. Anal. Biochem. 246:123‐132. | |
| Zhukov, A., Schurenberg, M., Jansson, O., Areskoug, D., and Buijs, J. 2004. Integration of surface plasmon resonance with mass spectrometry: Automated ligand fishing and sample preparation for MALDI MS Using a Biacore 3000 biosensor. J. Biomol. Tech. 15:112‐119. | |
| Key References | |
| Dhalluin et al., 2005. See above. | |
| Karlsson and Larsson. 2004. See above. | |
| Nordin et al., 2005. See above. | |
| Shuman et al., 2004. See above. | |
| Internet Resources | |
| http://www.biacore.com/lifesciences/index.html | |
| Details and animations of SPR technology, Biacore instruments, application notes, a reference database, guidelines to common immobilization chemistries, an immobilization and regeneration database, self‐training tools, and course listings are available. | |
| http://www.piercenet.com | |
| Maleimide coupling reagents are available from Pierce Chemical. |
GO TO THE FULL PROTOCOL:
PDF or HTML at Wiley Online Library
