Purification of Genomic DNA Using PureLink Silica Columns
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实验试剂
Sterile, DNase-free 1.5 ml microcentrifuge tubes for elution
Sterile water, pH 7-8.5, if you are using water for elution
实验设备
Microcentrifuge capable of centrifuging >10,000 x g
PureLink™ Spin Cartridge in Collection Tubes
实验材料
实验步骤
1. Remove a PureLink™ Spin Cartridge in a Collection Tube from the package.
2. Add the lysate with Binding Buffer (L3) and ethanol prepared to the PureLink™ Spin Cartridge.
3. Centrifuge the cartridge at 12,000 x g for 30 seconds at room temperature.
1. Add 500 µl Wash Buffer (W4) supplied in the kit to the cartridge.
3. Repeat Steps 1-2, once. Discard the flow through.
4. Add 500 µl Wash Buffer (W5) with ethanol (page 13) to the cartridge.
6. Repeat Steps 4-5, once. Discard the flow through.
1. Place the spin cartridge in a sterile 1.5-ml microcentrifuge tube.
2. Add 200 µl of Elution Buffer (E1) or sterile, distilled water (pH >7.0) to the cartridge.
1. Store the purified DNA at -20° C or use DNA for the desired downstream application.
注意事项
Follow the recommendations below to obtain the best results:
1. Perform all centrifugation steps at room temperature
2. Perform a 1 minute incubation step with Elution Buffer (E1) or water
3. Be sure to perform the recommended wash steps to obtain the best results
4. Always use sterile water, pH 7-8.5, if you are using water for elution
