Silinization of Slides硅化玻璃【Harvard University】

Cepko/Tabin Lab ,Harvard University

http://axon.med.harvard.edu/~cepko/protocol/mike/S6.html

Superfrost plus slides and standard slides can be silinized but I have found that superfrost slides work best so that protocol is outlined here.

Solutions

Reagents Needed

Superfrost Plus Slides

TESPA-3 aminopropyltrioxysilane (Sigma # A3648)

acetone

EtOH

Concentrated HCl

Procedure

• Dip a rack of superfrost plus slides in 2N HCl (200 ml Q 40 ml concentrated HCl)for 1 minute.

• Rinse the slides for 1 minute in Milli Q water.

• Rinse the slides in 100% Acetone for 1 minute.

• Incubate in 100% Acetone containing TESPA for 1 minute.

• Rinse briefly in 100% acetone.

• Rinse briefly in 95% EtOH followed by 50% EtOH,30% EtOH and several changes of Q.

• Air dry at room temperature overnight and then store at 4℃ dessicated until use.They keep for several months.