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Differentiation of Mouse Thymocytes in Fetal Thymus Organ Culture

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Most T cells develop in the thymus. Thymic development of T cells offers an excellent experimental system to pursue many issues of developmental biology, including
1.  lineage decisions, e.g., CD4+ T cells vs CD8+ T cells, and TCR-αβ+ T cells vs TCR-γδ+ T cells,
2.  clonal selection, e.g., positive selection of useful T-cell clones and negative selection of harmful T-cell clones, and
3.  cell migration, e.g., entry into and emigration from the thymus.
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