丁香实验_LOGO
登录
提问
我要登录
|免费注册
点赞
收藏
wx-share
分享

cDNA Microarray and Bioinformatic Analysis of Nuclear Factor-B Related Genes in Squamous Cell Carcinoma

互联网

453
Squamous cell carcinomas and several other cancers have been found to exhibit microarray expression profiles that include genes related to nuclear factor (NF)-κB, a signal activated transcription factor that is evolutionarily important in regulating early response gene programs to injury and infection. Inhibition of NF-κB by expression of a dominant negative signal phosphorylation site mutant of inhibitor-κB, IκBαM, under a tetracycline inducible promoter, established the role of NF-κB as an essential molecular switch modulating multiple genes important in the malignant phenotype. Bioinfomatic analysis of the promoter and coding region of IκBαM-modulated genes has enabled identification of new candidates with and without known NF-κB related motifs for validation and functional studies of their relationship to NF-κB. These studies illustrate how microarray data can be used to generate a hypothesis regarding regulation of genes by a specific signal transcription factor, and how genetic mutants and bioinformatic analysis can be used to analyze the relative importance of the regulatory molecule to expression of genes involved in the malignant phenotype.
提问
扫一扫
丁香实验小程序二维码
实验小助手
丁香实验公众号二维码
扫码领资料
反馈
TOP
打开小程序