Enzymes Acting on d-Amino Acid Containing Peptides
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Using a synthetic oligopeptide (d -Phe)4 , a microorganism Bacillus cereus DF4-B producing alkaline d -peptidase (ADP) was isolated. The enzymatic properties have been characterized; the enzyme showed d -stereospecific dipeptidyl aminopeptidase and endopeptidase activities. The enzyme was active toward (d -Phe) n , Boc-(d -Phe) n , (d -Phe) n methyl ester, d -Phe-NH2 , Boc-(d -Phe) n methyl ester, and Boc-(d -Phe) n tert -butyl ester, but not toward (d -Ala) n (n = 2–4), (d -Val)3 , and (d -Leu)2 .
