丁香实验_LOGO
登录
提问
我要登录
|免费注册
点赞
收藏
wx-share
分享

Identification of Protein-DNA Contacts with Dimethyl Sulfate: Methylation Protection and Methylation Interference

互联网

534
Dimethyl sulfate (DMS) is an effective and widely used probe for sequence-specific protein-DNA interactions. It is the only probe routinely used both for in vitro (methylation protection, methylation interference) and in vivo (DMS genomic footprinting) applications since it rapidly reacts with DNA at room temperature and readily penetrates intact cells (1 ). DMS predominantly methylates the 7-nitrogen of guanine and 3-nitrogen of adenine. Thus, reactivity with G residues occurs in the major groove and with A residues in the minor groove. In standard Maxam and Gilbert protocols (2 ), the methylated bases are subsequently converted to strand breaks and displayed on sequencing gels.
提问
扫一扫
丁香实验小程序二维码
实验小助手
丁香实验公众号二维码
扫码领资料
反馈
TOP
打开小程序