丁香实验_LOGO
登录
提问
我要登录
|免费注册
点赞
收藏
wx-share
分享

Generation and Culture of Mouse Embryonic Fibroblasts

互联网

510
The innate immune system is evolutionarily conserved and shared by a wide spectrum of cells, including epithelial cells, endothelial cells, fibroblasts, macrophages, dendritic cells, and lymphocytes. The extensive utilization of genetically manipulated animals in innate immunity studies has become the standard approach to confirm functional data acquired in cell lines. The easy generation and physiological relevance of mouse embryonic fibroblasts (MEFs) have made them a powerful tool in discovering novel signaling pathways, investigating regulatory networks, and exploring biochemical profiling of protein complexes involved in innate immune responses. Due to their extensive use, this chapter aims to provide a protocol for generating, maintaining, and storing primary MEFs for those who have minimal experience in animal models. Precautions and notes are integrated into the description of each step of the protocol for the benefits of minimizing unnecessary cross-referencing.
提问
扫一扫
丁香实验小程序二维码
实验小助手
丁香实验公众号二维码
扫码领资料
反馈
TOP
打开小程序