丁香实验_LOGO
登录
提问
提问
我要登录
|免费注册
丁香实验推荐阅读
Isolation, Purification, and Growth of Human Skeletal Muscle Cells

Skeletal muscle cells can be used in vitro for the study of myogenests, as well as in VIVO as gene-delivery vehicles for the therapy of muscle and nonmuscle diseases (1–9). These skeletal muscle cells are derived from muscle satellite cells, which he between the basal lamina and the sarcolemma of diffe ...

丁香实验推荐阅读
In Vitro Cellular Systems for Studying OC Function and Differentiation: Primary OC Cultures and the FLG 29.1 Model

Several pieces of evidence have shown that osteoclasts (OCs) are derived from progenitors originating from hemopoietic stem cells (1–3). More specifically, early OC precursors seem to be closely related to the colony-forming unit for granulocytes and macrophages (CFU-GM) (3–5. Howev ...

丁香实验推荐阅读
The Isolation of Osteoclasts from Human Giant Cell Tumors and Long-Term Marrow Cultures

The osteoclast is a large multinucleate cell formed from the fusion of mono-nuclear precursor cells of hemopoietic origin Unique markers of the osteoclast have been difficult to identify. Widely used techniques, such as histochemical location of tartrate-resistant acid phosphat ...

丁香实验推荐阅读
Isolation and Culture of Bone-Forming Cells (Osteoblasts) from Human Bone

The most conspicuous function of the osteoblast is the formation of bone. During phases of active bone formation, osteoblasts synthesize bone matrix and prime it for subsequent mineralization. Active osteoblasts are plump, cuboidal cells rich in organelles involved in the synthesis a ...

丁香实验推荐阅读
Human Tracheal Gland Cells in Primary Culture

For several years, tracheal gland cells have been cultured from different animal species, such as the cat (1), cow (2), and ferret (3). There are dlffer ences, however, in the structure and function of the various animal airways, rendering it difficult to extrapolate to humans. In this chapter, the auth ...

丁香实验推荐阅读
Genetic Analysis of Cystic Fibrosis Airway Epithelial Cells

More than 500 different mutations have been described to date on the cystic fibrosis (CF) gene (1). The most frequent mutation is the so-called ΔF508 mutation, which accounts for 30–50%;of CF chromosomes in southern European countries and for 50–80%; of CF chromosomes in the United States, Canada, A ...

丁香实验推荐阅读
Gene Transfer into Cystic Fibrosis Airway Epithelial Cells

Gene transfer into airway epithelial cells becomes a particularly motivating goal as far as cystic fibrosis (CF) is concerned. As mentioned in Chapter 15, approx 90% of deaths caused by this devastating disease are the result of infections of the respiratory tract owing to dysfunction of the Cl- t ...

丁香实验推荐阅读
Cystic Fibrosis Airway Epithelial Cell Culture

Cystic fibrosis (CF) is the most frequent (incidence around l/2500 live births) genetic cause of death among Caucasians. It is an autosomal recessive disorder compromising the secretory epithelia. Clinically, CF is a polymorphic disease showing abnormal functioning of the airways, t ...

丁香实验推荐阅读
FISH and Chips on the Internet

In this chapter we provide a unique collection of internet sites related to cytogenetics, FISH and FISH probes, array techniques, genome browsers, cytogenetic associations, (molecular) cytogenetic courses, the medical literature, genetics and biology, genetics and education, a ...

丁香实验推荐阅读
Array CGH: Opening New Horizons

Array comparative genomic hybridization (array CGH) enables the genome-wide detection of genomic copy number variations between test and reference samples. Genomic DNA from test and reference samples are differentially labeled with fluorochromes and cohybridized to DNA pro ...

丁香实验推荐阅读
Micro-CGH: Microdissection-Based Comparative Genomic Hybridization

Comparative genomic hybridization (CGH) is a well-established technique in the field of molecular cytogenetics for performing the comprehensive analysis of chromosomal imbalances of entire human genomes. Genomic DNA from a tested specimen (test DNA) and a normal one (reference DN ...

丁香实验推荐阅读
FISH in Food Microbiology

Due to continuous improvements in the FISH technique, it is now a very useful tool for studies in microbiology. Knowledge of the compositions and distributions of microorganisms in natural habitats plays a key role in safety and technological aspects of food microbiology. FISH can be used dir ...

丁香实验推荐阅读
FISH on Plant Chromosomes

FISH, which allows the mapping of DNA sequences directly onto chromosomes, has revolutionized plant molecular cytogenetics research since it was first adapted from mammalian research. Repetitive DNA sequences can generate unique FISH patterns on individual chromosomes for ka ...

丁香实验推荐阅读
FISH Targeting of Chromosomes and Subchromosomal Regions in Yeast

Yeasts represent powerful genetic model organisms, due in part to their tiny genomes with very little repetitive DNA. However, one drawback of this feature is an ill-defined metaphase chromosome structure. Fluorescence in situ hybridization has overcome this cytological disadva ...

丁香实验推荐阅读
FISH on Insect Cells Transfected with Heterologous DNA: A Single-Cell-Directed Approach

Insect cells transfected with heterologous DNA can be used to assess the functional properties of genes that have been isolated or subjected to in vitro mutagenesis. An example of such a cell system is embryonic cells derived from Drosophila melanogaster. These cells can be transfected with s ...

丁香实验推荐阅读
Animal Probes and ZOO-FISH

The invention of cross-species chromosome painting (ZOO-FISH) represents the most significant technical breakthrough in animal cytogenetics since the introduction of chromosomal banding techniques in the late 1960s and early 1970s. It has made it possible to compare the karyoty ...

丁香实验推荐阅读
Three-Dimensional Interphase Analysis Enabled by Suspension FISH

An established method for performing the entire FISH procedure in suspension instead of on a slide (the usual approach) is described. This so-called suspension FISH (S-FISH) technique has opened up new possibilities for the analysis of the shape and internal structure of the human interpha ...

丁香实验推荐阅读
Interphase FISH: Detection of Intercellular Genomic Variations and Somatic Chromosomal Mosaicism

Interphase fluorescence in situ hybridization (I-FISH) beautifully exemplifies what a molecular cytogenetic technique can offer in contrast to conventional cytogenetic approaches: the analysis of chromosomal DNA at all stages of the cell cycle. It has made it possible to study chro ...

丁香实验推荐阅读
Parental Origin Determination FISH: Pod-FISH

Except for when individuals have variations in the pericentric heterochromatic chromosomal regions (including acrocentric short arms), it is not possible to distinguish between homologous chromosomes at a single-cell level. Due to this limitation, various questions of scien ...

丁香实验推荐阅读
Quantitative DNA Fiber Mapping

Several hybridization-based methods that are used to delineate single-copy or repeated DNA sequences over larger genomic intervals take advantage of the increased resolution and sensitivity of free chromatin, i.e., chromatin released from interphase cell nuclei. Quantitati ...

提问
扫一扫
丁香实验小程序二维码
实验小助手
丁香实验公众号二维码
关注公众号
反馈
TOP
打开小程序