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Generation of Lung Epithelial-Like Tissue from hESC by AirLiquid Interface Culture

Human embryonic stem cells (hESCs) have the capacity to differentiate in vivo and in vitro into cells from all three germ lineages. The in vitro generation of lung cells and tissues from hESCs creates opportunities for fundamental research, drug development or cell-replacement therapy. In ...

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Hepatic Maturation of hES Cells by Using a Murine Mesenchymal Cell Line Derived from Fetal Livers

Hepatocytes derived from human embryonic stem (hES) cells are a potential cell source for regenerative medicine. However, the successful differentiation of hES cells into mature hepatocytes has been difficult to achieve because the definitive mechanisms governing hepatocyte ...

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Multistage Hepatic Differentiation from Human Induced Pluripotent Stem Cells

Efficient hepatic differentiation technologies designed for patient specific induced pluripotent stem (iPS) cells may provide an unlimited hepatocyte source which can be utilized in drug screening, disease modeling, and cell therapy. This chapter describes the methods we use to d ...

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Deriving Metabolically Active Hepatic Endoderm from Pluripotent Stem Cells

The human liver is a vital organ within the body and plays a major role in normal homeostasis. The “work horse” of the liver, termed the “hepatocyte,” is estimated to make up approximately 70–80% of the liver’s mass. Therefore, the study of hepatocyte biology has an important role to play in medicine and the drug ...

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Chondrogenic Differentiation of hESC in Micromass Culture

Human adult cartilage has limited capacity for self-renewal. Accordingly, repair of cartilage tissue damaged as a result of acute traumatic injury or via chronic wear or degenerative disease, such as arthritis, is a major clinical problem. Human embryonic stem cells (hESCs) could provide ...

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Adipogenic Differentiation of Human Induced Pluripotent Stem Cells

Human induced pluripotent stem (iPS) cells are promising sources of disease modeling and regenerative medicine. However, differentiation properties of human iPS cells to specific lineages still remain to be fully clarified. Here, we describe a protocol for differentiating human i ...

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Generation of Multipotent CD34+CD45+ Hematopoietic Progenitors from Human Induced Pluripotent Stem Cells

Human embryonic stem cells (hESCs) and patient-specific human induced pluripotent stem cells (hiPSCs) are valuable reagents for studying the earliest stages of hematopoietic genesis and for modeling the developmental basis of hematologic disorders, and they may also have the pote ...

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Differentiation of Human Embryonic and Induced Pluripotent Stem Cells into Blood Cells in Coculture with Murine Stromal Cells

The establishment of human embryonic stem cell (hESC) lines, as well as the recent induced pluripotent stem cells (hiPSC), has greatly expanded our knowledge about the early development in human ontogeny. In the past decade, hESCs and hiPSCs have been proven excellent tools in characterizat ...

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Differentiation of Endothelial Cells from Human Embryonic Stem Cells and Induced Pluripotent Stem Cells

Endothelial cells line the entire circulatory system and form the interface between the blood vessel intima and the circulating red blood cells. Endothelial cells are crucial to the proper function of the circulatory system and tissue viability, including their roles in coagulation, f ...

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Feeder-Independent Maintenance of Human Embryonic Stem Cells and Directed Differentiation into Endothelial Cells Under Hypoxic Condition

Human embryonic stem cells (hESCs) represent a promising source of cells for modern regenerative medicine due to their highly self-renewal capability and pluripotency to differentiate into almost all cell types from three different germ layers. Recent advances in the stem-cell field ...

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Feeder-Free Culture for High Efficiency Production of Subculturable Vascular Endothelial Cells from Human Embryonic Stem Cells

Conventional methods for vascular endothelial differentiation of human embryonic stem (hES) cells had suffered from subculture incompetence of the final products due to dominant expansion of contaminating pericytic components. We have overcome this problem by adding a “hemat ...

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In Vitro Two-Dimensional Endothelial Differentiation of Human Embryonic Stem Cells

Pluripotent human embryonic stem cells (hESCs) comprise of cells from all three germ layers in vivo. They have been proved to differentiate in vitro into a variety of cell lineages. Endothelial cells derived from hESCs could potentially contribute to cellular treatment of vascular disea ...

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Generation of Induced Pluripotent Stem Cells from Human Amnion Cells

Induced pluripotent stem (iPS) cells have been generated through nuclear reprogramming of somatic cells via retrovirus- or lentivirus-mediated transduction of exogenous reprogramming factors OCT3/4, SOX2, KLF4, and c-MYC. The extraembryonic amnion is considered to be a promis ...

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Generation of iPS Cells from Human Skin Biopsy

The reprogramming of human somatic cells to induced pluripotent stem (iPS) cells has offered the �opportunity to derive patient-specific cells with embryonic stem cell (ESC) properties. Human iPS cells demonstrate the ability to self-renew and to differentiate into any cell type of the b ...

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Induced Pluripotent Stem Cells (iPSC) from Cord Blood CD133+ Cells Using Oct4 and Sox2

Induced pluripotent stem cells (iPSCs) provide an invaluable resource for regenerative medicine to repair tissues damaged through disease or injury. Although human iPSCs have been generated using different type of somatic cells, such as skin fibroblasts and keratinocytes, neural ...

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Generation of Nonviral Integration-Free Induced Pluripotent Stem Cells from Plucked Human Hair Follicles

Human induced pluripotent stem cells (hiPSCs) provide a unique experimental reagent for dissecting the complex transcriptional, regulatory, and epigenetic mechanisms of pluripotency, as well as for studying normal and diseased human development. However, the utility of curre ...

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Retroviral Vector-Based Approaches for the Generation of Human Induced Pluripotent Stem Cells from Fibroblasts and Keratinocytes

The ability of somatic cells to be induced to pluripotency by ectopic expression of defined transcription factors has altered the course of research in developmental biology and regenerative medicine. Somatic cell reprogramming has now been performed with numerous somatic sourc ...

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Human Mesenchymal Stem Cells and iPS Cells (Preparation Methods)

Mesenchymal stem cells (MSCs) are adult stem cells which show differentiation capabilities toward various cell lineages, and the MSCs can be cryopreserved for a long term without noticeable loss of these capabilities. We have used bone marrow–derived MSCs for treatments of patients with ...

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Derivation of Human Induced Pluripotent Stem Cells on Autologous Feeders

One of the possible applications of human induced pluripotent stem (iPS) cells is its usage for transplantation (Takahashi K et al. Cell 131:861–872, 2007; Yu J et al. Science 318:1917–1920, 2007; Park IH et al. Nature 451:141–146, 2008; Lowry WE et al. Proc Natl Acad Sci USA 105:2883–2888, 2008; Morita S et al. Gene ...

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Generation of Human Induced Pluripotent Stem Cells from Endoderm Origin Cells

Human induced pluripotent stem (iPS) cells have been derived mostly from cells originating from �mesoderm and in a few cases from ectoderm. This has prevented comprehensive comparative investigations of the quality of human iPS cells of different origins. We have recently reported for t ...

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