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丁香实验推荐阅读
Analyzing the Angiogenic Potential of Gr-1+CD11b+ Immature Myeloid Cells from Murine Wounds

Analysis of tissue repair and regeneration in a variety of organisms has demonstrated that stem and progenitor cells play a critical role in the healing and regenerative response. In particular, during cutaneous wound healing bone marrow-derived cells are recruited to the site of injury in ...

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Live Imaging for Studying Asymmetric Cell Division in the C. elegans Embryo

Asymmetric cell division is essential during development to generate cell diversity and throughout adult life to maintain tissue homeostasis. For instance, many types of stem cells must divide asymmetrically to maintain their self-renewal capacities. Furthermore, recent stu ...

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Transcriptome Analysis of Drosophila Neural Stem Cells

In Drosophila, the central nervous system is populated by a set of asymmetrically dividing neural stem cells called neuroblasts. Neuroblasts are derived from epithelial or neuroepithelial precursors, and divide along their apico-basal axes to produce a large apical neuroblast and a s ...

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Following the Fate of Neural Progenitors by Homotopic/Homochronic Grafts in Xenopus Embryos

The neural plate consists of neuroepithelial cells that serve as progenitors for the mature central nervous system. The neural plate is a highly regionalized structure, harboring neural progenitors with different programs of differentiation, due to signaling or intrinsic diffe ...

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Reconstitution of the Central Nervous System During Salamander Tail Regeneration from the Implanted Neurospheres

Urodele amphibians such as axolotl are well known for their regenerative potential of the damaged central nervous system structures. Upon tail amputation, neural stem cells behind the amputation plane undergo self-renewing divisions and contribute to the functional spinal cord in ...

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Clonal and Lineage Analysis of Melanocyte Stem Cells and Their Progeny in the Zebrafish

The study of melanocyte biology in the zebrafish presents a highly tractable system for understanding fundamental principles of developmental biology. Melanocytes are visible in the transparent embryo and in the mature fish following metamorphosis, a physical transformation ...

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FACS Analysis of the Planarian Stem Cell Compartment as a Tool to Understand Regenerative Mechanisms

Planarians provide a relatively simple model system in which to study stem cell dynamics and regenerative phenomena. As with other systems understanding the dynamics of stem cell and stem cell progeny is crucial in order to get at the molecular mechanisms orchestrating stem cell biology. Pl ...

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Identification of Oocyte Progenitor Cells in the Zebrafish Ovary

Zebrafish breed year round and females are capable of producing thousands of eggs during their lifetime. This amazing fecundity is due to the fact that the adult ovary, contains premeiotic oocyte progenitor cells, called oogonia, which produce a continuous supply of new oocytes throughout ...

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Labeling Primitive Myeloid Progenitor Cells in Xenopus

In Xenopus the first blood cells to differentiate in the embryo are the primitive myeloid lineages, which arise from the anterior ventral blood islands during the neurula stages. Primitive myeloid cells (PMCs) will give rise to the embryonic pool of neutrophils and macrophages, a highly migr ...

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Tol2-Mediated Gene Transfer and In Ovo Electroporation of the Otic Placode: A Powerful and Versatile Approach for Investigating Embryonic Development

The vertebrate inner ear is composed of several specialized epithelia containing mechanosensory “hair” cells, sensitive to sound and head movements. In mammals, the loss of hair cells for example during aging or after noise trauma is irreversible and results in permanent sensory defici ...

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Isolation and Expansion of Endothelial Progenitor Cells Derived from Mouse Embryonic Stem Cells

The unlimited differentiation and proliferation capacity of embryonic stem cells represents a great resource for regenerative medicine. Here, we describe a method for differentiating, isolating, and expanding endothelial cells (ECs) from mouse embryonic stem cells (mESCs). F ...

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Functional Purification of Human and Mouse Mammary Stem Cells

Normal and tumor stem cells are present in rare quantities in tissues and this has historically represented a major hurdle to in-depth investigations of their biology. In the case of the mammary gland, the relative promiscuity of the immunophenotypical markers described in several studi ...

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Isolation of Adult Stem Cells and Their Differentiation to Schwann Cells

Peripheral nerve injuries are an economic burden for society in general and despite advanced microsurgical reconstruction of the damaged nerves the functional result is unsatisfactory with poor sensory recovery and reduced motor functions (Wiberg and Terenghi, Surg Technol Int ...

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Three-Dimensional In Vitro Culture Techniques for Mesenchymal Stem Cells

In recent years there has been a growing interest in culturing adherent cells using three-dimensional (3D) techniques, rather than more conventional 2D culture methods. This interest emerges from the realization that growing cells on plastic surfaces cannot truly re-create 3D in vivo c ...

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Isolation, Culture, and Potentiality Assessment of Lung Alveolar Stem Cells

The cellular and molecular elements involved in the turnover and regeneration of the lung alveolar epithelium remain largely unknown (Kim, Am J Physiol Lung Cell Mol Physiol 293:L1092–1098, 2007). Isolation and characterization of putative stem cells with limited and nonspecific mar ...

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Terminal Differentiation of Human Epidermal Stem Cells on Micro-patterned Substrates

Extracellular signals play a central role in coordinating the growth and differentiation of epidermal stem cells. This protocol describes a technique for quantitatively examining the influence of extracellular matrix (ECM) interactions on keratinocyte terminal differen ...

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Isolation of Satellite Cells from Single Muscle Fibers from Young, Aged, or Dystrophic Muscles

Skeletal muscle contains an identified resident stem cell population called the satellite cells. This cell is responsible for the majority of the postnatal growth and regenerative potential of skeletal muscle. Other cells do contribute to skeletal muscle regeneration and in cultur ...

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Derivation of Human Embryonic Stem Cell Lines from Poor Quality Embryos

A serious shortcoming in the derivation of human embryonic stem cell (hESC) lines has been the availability of human embryos. About 60% of human embryos generated by in vitro fertilization (IVF) fail to develop normally and are unusable for fertility treatment. Such embryos often retain suff ...

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Characterization and Gene Expression Profiling of Five Human Embryonic Stem Cell Lines Derived in Taiwan

Human embryonic stem cell (hESC) lines have been derived from the inner cell mass of blastocysts. Five hESC lines have been derived from 32 discarded blastocysts in Taiwan, and these lines have since been continuously cultured on mitotically inactivated mouse embryonic fibroblasts as fe ...

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Analysis of LINE-1 Expression in Human Pluripotent Cells

Half of the human genome is composed of repeated DNA, and some types are mobile within our genome (transposons and retrotransposons). Despite their abundance, only a small fraction of them are currently active in our genome (Long Interspersed Element-1 (LINE-1), Alu, and SVA elements). LINE-1 or L1 ...

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