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丁香实验推荐阅读
Methods for Culture of Human Corneal and Conjunctival Epithelia

The surface of the eye is exposed to the outside world and is, thus, subject to surface abrasion, infections, and drying, cicatrizing diseases. Availability of in vitro methods for culture of the human corneal and conjunctival epithelia, which cover the ocular surface, is therefore important in ...

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Culture of Choroid Plexus Epithelial Cells and In Vitro Model of BloodCSF Barrier

Chemical homeostasis in the extracellular fluid of the central nervous system (CNS) is maintained by two brain barrier systems, i.e., the blood–brain barrier (BBB) that separates the blood circulation from brain interstitial fluid and the blood–cerebrospinal fluid barrier (BCB) that ...

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Viral Transformation of Epithelial Cells

Approximately 18% of human cancers have a viral etiology and the majority of these involve transformation of epithelial cells. Viral proteins transform epithelia by inducing alterations in the normal cell growth and differentiation pathways through the targeting of host proteins. ...

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Generation of Human Cell Lines Using Lentiviral-Mediated Genetic Engineering

Even now, most human cell lines used in research are derived from tumor cells. They are still widely used because they grow well in vitro and so far have helped answering several basic biological questions. However, as modern biology moves into more sophisticated areas, scientists now need human c ...

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Analysis of Tissue Interactions in Ectodermal Organ Culture

The morphogenesis of ectodermal organs is regulated by epithelial mesenchymal interactions mediated by conserved signaling molecules. Analyzing the roles of these molecules will increase our understanding of mechanisms regulating organogenesis, and organ culture meth ...

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Urothelial Cell Culture: Stratified Urothelial Sheet and Three-Dimensional Growth of Urothelial Structure

Urothelial cells line the urinary tract, including the renal pelvis, ureters, bladder, superior urethra, and the central ducts of the prostate. They are highly specialized epithelial cell types possessing unique features, imparting important functional roles in the urinary system. ...

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Primary Culture and Propagation of Human Prostate Epithelial Cells

Basic and translational (or preclinical) prostate cancer research has traditionally been conducted with a limited repertoire of immortalized cell lines, which have homogeneous phenotypes and have adapted to long-term tissue culture. Primary cell culture provides a model system ...

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Classical Human Epidermal Keratinocyte Cell Culture

It has been more than 30 years since the serial cultivation of human keratinocytes in monolayer culture was first described by Rheinwald and Green. Initially, isolation of primary keratinocytes from disaggregated human skin tissue and subsequent propagation was promoted through use ...

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Isolation and Culture of Alveolar Epithelial Type I and Type II Cells from Rat Lungs

The pulmonary alveolar epithelium, comprised of alveolar Type I (TI) and Type II (TII) cells, covers more than 99% of the internal surface area of the lungs. The study of isolated and cultured alveolar epithelial TI and TII cells has provided a large amount of information about the functions of both cell t ...

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Mouse Epidermal Keratinocyte Culture

Cultured mouse epidermal keratinocytes provide a powerful model for studying epidermal proliferation, differentiation, pathogenesis, and oncogenic transformation. Primary mouse keratinocytes can be isolated from newborn mice, and their growth and differentiation c ...

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Three-Dimensional Culture of Human Breast Epithelial Cells: The How and the Why

Organs are made of the organized assembly of different cell types that contribute to the architecture necessary for functional differentiation. In those with exocrine function, such as the breast, cell–cell and cell–extracellular matrix (ECM) interactions establish mechanist ...

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Three-Dimensional Cultures of Mouse Mammary Epithelial Cells

The mammary gland is an ideal “model organism” for studying tissue specificity and gene expression in mammals: it is one of the few organs that develop after birth and it undergoes multiple cycles of growth, differentiation and regression during the animal’s lifetime in preparation for the imp ...

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Isolation, Characterization, and Reaggregate Culture of Thymic Epithelial Cells

The thymus organ is composed of a three-dimensional (3D) network of adjoining epithelium and stromal cells. Bone marrow-derived T cell precursors, upon entering the thymus, interact with and migrate through this cellular network as they differentiate and mature. An essential compone ...

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Enrichment and Clonal Culture of Hepatic Stem/Progenitor Cells During Mouse Liver Development

Liver regenerates after hepatectomy or chemical-induced injury. In contrast to cells in other tissues that can regenerate, mature cells (hepatocytes), but not undifferentiated stem cells, are mainly responsible for acute liver regeneration. Liver stem cells take part in liver rege ...

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Long-Term Culture and Coculture of Primary Rat and Human Hepatocytes

The liver is the largest internal organ in mammals, serving a wide spectrum of vital functions. Loss of liver function due to drug toxicity or viral infection is a major cause of death in the United States. The development of Bioartificial Liver (BAL) devices and the demand for pharmaceutical and cosm ...

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Tissue Culture Correlational Study of Genetic Cholangiopathy of Autosomal Recessive Polycystic Kidney Disease

Cholangiocytes are epithelial cells that line the biliary tract and are also known as biliary epithelial cells (BECs). In vitro culture studies of BECs in correlation with tissue section examination may give us a comprehensive analysis of biliary tract diseases. Herein, we discuss genetic ...

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Primary Mouse Small Intestinal Epithelial Cell Cultures

The intestinal epithelium is the most rapidly self-renewing tissue in adult mammals. We have recently shown that Lgr5 (Leucine-rich repeat-containing G protein-coupled receptor) is expressed in intestinal stem cells by an in vivo genetic lineage tracing strategy. In the past, extensi ...

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Progress Towards Drosophila Epithelial Cell Culture

Drosophila epithelial research is at the forefront of the field; however, there are no well-characterized epithelial cell lines that could provide a complementary in vitro model for studies conducted in vivo. Here, a protocol is described that produces epithelial cell lines. The method us ...

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Isolation, Growth, and Characterization of Human Renal Epithelial Cells Using Traditional and 3D Methods

The kidney is a highly heterogeneous organ that is responsible for fluid and electrolyte balance. Much interest is focused on determining the function of specific renal epithelial cells in humans, which can only be accomplished through the isolation and growth of nephron segment-speci ...

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Human Female Reproductive Tract Epithelial Cell Culture

The female reproductive system is a complex system. Epithelia of the female reproductive system including the ovaries, the oviduct, and the uterus are important sites for follicular development, ovulation, fertilization, implantation, and embryo development. They are also able to s ...

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