Infusion of bone marrow or cytokine mobilized stem cells can be associated with severe and potentially life-threatening toxicities. The etiology of the toxicities can be related to volume of infusion, dimethylsulfoxide (DMSO) or bacterial or viral infection of the infused cell produc ...

An important factor contributing to hematopoietic stem cell (HSC) mobilization is the ability of mobilizing cytokines and chemotherapy to disturb the cellular components of HSC niches, particularly osteoblasts and their progenitors, and to inhibit the production of HSC support ...

It has long been recognized that single therapies, such as G-CSF, have a limited capacity to mobilize hematopoietic progenitor cells from the bone marrow. As a consequence in ∼20% of patients insufficient numbers of HPCs are mobilized to perform a bone marrow transplant. Recent studies have sho ...

Blood vessel formation plays a key role in both physiologic and pathologic tissue growth and healing. Thus, a thorough understanding of the mechanisms underlying neovascularization will translate into innovative clinical treatment strategies for a wide variety of disease proce ...

Modern apheresis generally results in a mononuclear cell product that is highly enriched for hematopoietic stem and progenitor cells when performed on patients who have mobilized with myeloid growth factors, CXCR4 antagonists or upon recovery from cytotoxic chemotherapy. The dur ...

Adverse reactions may occur during the Hematopoietic Progenitor Cells via Apheresis (HPC-A) �collection, although they are rare. They can be associated with physical, psychological, or environmental factors. The most common complications are hypocalcemia related to citrate t ...

Hematopoietic progenitor cells can be mobilized from the bone marrow microenvironment into the peripheral blood following treatment of patients with myeloid cytokines (GCSF, GMCSF, IL3), a CXCR4 antagonist (Plerixafor) and/or following a hematopoietic recovery from cytotox ...

Hematopoietic Progenitor Cell (HPC) Apheresis generally results in a mononuclear cell product that is highly enriched for hematopoietic stem and progenitor cells when performed on autologous patients in whom autologous stem cell transplant is planned who have been mobilized with ...

Autologous hematopoietic stem cell transplantation is the standard treatment for a wide variety of malignancies. At present, most hematopoietic progenitor/stem cell (HPC) collections are collected from the peripheral blood via leukapheresis following chemotherapy and/ ...

Hematopoietic stem and progenitor mobilization has revolutionized the field of hematopoietic transplantation. Currently, hematopoietic grafts acquired from the peripheral blood of patients or donors treated with granulocyte-colony stimulating factor (G-CSF) are the ...

Cytokine mobilized peripheral blood stem cells are the preferred source of stem cells in autologous stem cell transplantation and have virtually replaced bone marrow as the stem cell source. In recent years, a dramatic increase has been reported in the use of peripheral blood stem cells for all ...

Allogeneic donor blood cells and autologous peripheral blood leukocytes (PBL), obtained following �clinical mobilization procedures, are routinely used as a major source of hematopoietic stem and progenitor cells (HSPC) for transplantation protocols. It is, therefore, esse ...

The course of various pathological conditions relies on the mobilization of stem cells and partially differentiated progenitor cells. Bone marrow transplantation studies have demonstrated that medullary hematopoietic and endothelial progenitors can undergo mobiliz ...

Hematopoietic stem/progenitor cell mobilization can be achieved by a variety of bone marrow niche modifications, although efficient mobilization requires simultaneous expansion of the stem/progenitor cell pool and niche modification. Many of the mechanisms involved in G-C ...

While many murine models have been developed to study adult disease, animal research focused on neonatal and pediatric medicine has been limited by the small size of the mouse pups. Several transplantation, injection, and implantation systems have been used to study the function and role of va ...

Airway epithelial cell biology has been greatly advanced by studies of genetically defined and modified mice; however it is often difficult to isolate, manipulate, and assay epithelial cell-specific responses in vivo. In vitro proliferation and differentiation of mouse airway epi ...

Human airway epithelial (hAE) cell cultures are instrumental for studying basic and applied aspects of respiratory tract biology, disease, and therapy. When primary epithelial cells from the human nasal passages or tracheo-bronchial airways are grown on porous supports at an air-liq ...

Establishment of primary and immortalized cultures of many cell types has facilitated efforts to understand the signals involved in proliferation and differentiation and yielded tools to rapidly assay new molecules targeting specific receptor pathways. Taste cells are speci ...

The central cell type involved in the initial perception of odors and transduction of the sensory signal are the olfactory receptor neurons (ORNs) located in the olfactory neuroepithelium of the nasal cavities. The olfactory epithelium is a unique system similar to the neuroepithelium of ...

Primary human oral epithelial cells are readily available and have been recently employed for tissue engineering. These cells are currently being widely utilized in multiple research efforts, ranging from the study of oral biology, mucosal immunity, and carcinogenesis to stem cell bi ...