丁香实验_LOGO
登录
提问
提问
我要登录
|免费注册
丁香实验推荐阅读
Electrical Recordings from the Accessory Olfactory Bulb in VNO-AOB Ex Vivo Preparations

Electrical recordings from individual accessory olfactory bulb neurons allow exploration of the functional properties of this important pheromonal processing circuit. Several approaches to performing such recordings have been used. Here, we describe ex vivo methods that we h ...

丁香实验推荐阅读
The Electrovomeronasogram: Field Potential Recordings in the Mouse Vomeronasal Organ

Mammalian vomeronasal neurons (VSNs) located in the sensory epithelium of the vomeronasal organ (VNO) detect and transduce molecular cues emitted by other individuals and send this information to the olfactory forebrain. The initial steps in the detection of pheromones and other che ...

丁香实验推荐阅读
Whole-Mount Imaging of Responses in Mouse Vomeronasal Neurons

Imaging permits the visualization of neural activity from the whole-mount vomeronasal sensory epithelium with single-cell resolution. The preparation preserves an intact tissue environment, enabling the robust detection of cellular responses upon chemical stimulation ...

丁香实验推荐阅读
Live Cell Calcium Imaging of Dissociated Vomeronasal Neurons

Sensory neurons in the vomeronasal organ (VNO) are thought to mediate a specialized olfactory response. Currently, very little is known about the identity of stimulating ligands or their cognate receptors that initiate neural activation. Each sensory neuron is thought to express 1 of app ...

丁香实验推荐阅读
Electroantennogram and Single Sensillum Recording in Insect Antennae

Electrophysiology is an invaluable technique to quickly and quantitatively assess the response of the olfactory system to odor stimuli. For measuring the response of the insect antenna, two basic techniques exist, electroantennography and single sensillum recording. Here, we de ...

丁香实验推荐阅读
A Protocol for Heterologous Expression and Functional Assay for Mouse Pheromone Receptors

Innate social behaviors like intermale aggression, fear, and mating rituals are important for survival and propagation of a species. In mice, these behaviors have been implicated to be mediated by peptide pheromones that are sensed by a class of G protein-coupled receptors, vomeronasal re ...

丁香实验推荐阅读
Identification of Chemosensory Receptor Genes from Vertebrate Genomes

Chemical senses are essential for the survival of animals. In vertebrates, mainly three different types of receptors, olfactory receptors (ORs), vomeronasal receptors type 1 (V1Rs), and vomeronasal receptors type 2 (V2Rs), are responsible for the detection of chemicals in the environ ...

丁香实验推荐阅读
Genetic Manipulation to Analyze Pheromone Responses: Knockouts of Multiple Receptor Genes

Gene targeting in the mouse is an essential technique to study gene function in vivo. Multigene families encoding vomeronasal receptor (VR) type 1 and type 2 consist of ~300 intact genes, which are clustered at multiple loci in the mouse genome. To understand the function of VRs and neurons expressi ...

丁香实验推荐阅读
Analysis of Ascarosides from Caenorhabditis elegans Using Mass Spectrometry and NMR Spectroscopy

The nematode Caenorhabditis elegans secretes a family of water-soluble small molecules, known as the ascarosides, into its environment and uses these ascarosides in chemical communication. The ascarosides are derivatives of the 3,6-dideoxysugar ascarylose, modified with di ...

丁香实验推荐阅读
Chemical Analysis of Aquatic Pheromones in Fish

Pheromones are chemicals that pass between members of the same species that have inherent meaning. In the case of fish, pheromones are water-soluble and found in low concentrations. As such, sensitive and selective methods are needed to separate and analyze these pheromones from an environ ...

丁香实验推荐阅读
Murine Nonvolatile Pheromones: Isolation of Exocrine-Gland Secreting Peptide 1

Our search for a substance recognized by the vomeronasal neurons revealed that the extra-orbital lacrimal gland (ELG) isolated from adult male mice produced the male-specific peptide pheromone exocrine gland-secreting peptide 1 (ESP1). The following protocol reveals how ESP1 may be ...

丁香实验推荐阅读
Pheromones in the Fruit Fly

The identification of pheromones (chemical communication cues) is critical to our understanding of complex social behavior in insects and other animals. In this chapter, we describe analytical methods for the purification of lipid pheromones by thin layer chromatography and the qu ...

丁香实验推荐阅读
Female Moth Pheromones

The sex pheromone, a volatile secreted by a female moth, is stored in the pheromone gland and can be easily extracted with hexane. The extract is effectively analyzed using a gas chromatography combined with an electro-antennogram detector (GC-EAD) and a mass spectrometry (GC-MS), both of which ...

丁香实验推荐阅读
Using Somatic-Cell Nuclear Transfer to Study Aging

In mammals, a diploid genome following fertilization of haploid cells, an egg, and a spermatozoon is unique and irreproducible. This implies that the generated unique diploid genome is doomed with the individual’s inevitable demise. Since it was first reported in 1997 that Dolly the sheep had b ...

丁香实验推荐阅读
The Use of Calorie Restriction Mimetics to Study Aging

Calorie restriction (CR) has a variety of effects on extending lifespan and delaying the onset of age-related diseases, and it is accepted as the only established experimental antiaging intervention. Several pharmacological agents that can replicate the beneficial effects of CR, ca ...

丁香实验推荐阅读
Aging Studies in Drosophila Melanogaster

Drosophila is a genetically tractable system ideal for investigating the mechanisms of aging and developing interventions for promoting healthy aging. Here we describe methods commonly used in Drosophila aging research. These include basic approaches for preparation of diets a ...

丁香实验推荐阅读
Methods for Creating Mutations in C. elegans That Extend Lifespan

The principle of commonly used methods to create mutations in the nematode Caenorhabditis elegans (C. elegans) is straightforward. In general, worms are exposed to a dose of mutagen resulting in DNA damages and mutations. Screening the progeny of the mutagenized animals for a certain pheno ...

丁香实验推荐阅读
Studying the Replicative Life Span of Yeast Cells

The budding yeast Saccharomyces cerevisiae is a useful model for elucidating the pathways that control life span and the influence of environmental factors, such as calorie restriction (CR). For 75 years, CR has been studied for its ability to delay diseases of aging in mammals, from cancer to car ...

丁香实验推荐阅读
Cell Sorting of Young and Senescent Cells

Cellular senescence is the irreversible loss of proliferative potential and is accompanied by a number of phenotypic changes. First described by Hayflick and Moorhead in 1961, it has since become a popular model to study cellular aging. The replicative lifespan of human fibroblasts is het ...

丁香实验推荐阅读
Analysis of Biomarkers of Caloric Restriction in Aging Cells

Caloric restriction (CR) has been extensively documented for its profound role in effectively extending maximum lifespan in many different species. However, the accurate mechanisms, especially at the cellular level, for CR-induced aging delay are still under intense investigat ...

提问
扫一扫
丁香实验小程序二维码
实验小助手
丁香实验公众号二维码
关注公众号
反馈
TOP
打开小程序