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丁香实验推荐阅读
Visual DNA Detection and SNP Genotyping Using Asymmetric PCR and Split DNA Enzymes

We describe a method to detect DNA sequences visually through a color change reaction using DNAzymes. We successfully applied the assay for the detection of Salmonella and Mycobacterium DNA, as well as for genotyping single base differences from within human genomic DNA samples. Our appro ...

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DNA Detection by Cascade Enzymatic Signal Amplification

Although many approaches based on template replication were developed and applied in DNA detection, cross-contamination from amplicons is always a vexing problem. Thus, signal amplification is preferable for DNA detection due to its low risk of cross-contamination from amplicons. ...

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Loop-Mediated Isothermal Amplification Method for a Differential Identification of Human Taenia Tapeworms

Loop-mediated isothermal amplification (LAMP), which employs a Bst DNA polymerase with strand-displacement activity and four primers (two inner primers and two outer primers) recognizing six distinct regions on the target DNA, is a highly sensitive, specific, simple, and rapid nucl ...

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Rapid Detection of Brucella spp. Using Loop-Mediated Isothermal Amplification (LAMP)

Brucella spp. are facultative intracellular bacteria that cause zoonotic disease of brucellosis worldwide. Livestock that are most vulnerable to brucellosis include cattle, goats, and pigs. Brucella spp. cause serious health problems to humans and animals and economic losses to the ...

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Detection of rpoB Gene Mutations Using Helicase-Dependent Amplification

For patients infected with tuberculosis, detection of rpoB gene mutations is critical for diagnosing drug-resistant strains of the causative pathogen, Mycobacterium tuberculosis (MTB). Traditional approaches to drug resistance include culture, which is very slow. Recently ...

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Detection of Mutation by Allele-Specific Loop-Mediated Isothermal Amplification (AS-LAMP)

For effective control of pathogen-transmitting mosquitoes, precise surveillance data of mosquito distribution are essential. Recently, an increase of insecticide resistance due to the kdr mutation in Anopheles gambiae, a mosquito that transmits the malaria parasite, has been r ...

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SNP Analysis Using a Molecular Beacon-Based Operating Cooperatively (OC) Sensor

Analysis of single-nucleotide polymorphisms (SNPs) is important for diagnosis of infectious and genetic diseases, for environment and population studies, as well as in forensic applications. Herein is a detailed description to design an “operating cooperatively” (OC) sensor for ...

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Detection of SNP-Containing Human DNA Sequences Using a Split Sensor with a Universal Molecular Beacon Reporter

Hybridization-based techniques have been extensively employed for the analysis of specific DNA/RNA sequences. Herein, we describe highly specific inexpensive smart hybridization-based sensor that takes advantage of a universal molecular beacon probe as a fluorescent rep ...

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A New, Multiplex, Quantitative Real-Time Polymerase Chain Reaction System for Nucleic Acid Detection and Quantification

Quantitative real-time polymerase chain reaction (qPCR) has emerged as a powerful investigative and diagnostic tool with potential to generate accurate and reproducible results. qPCR can be designed to fulfil the four key aspects required for the detection of nucleic acids: simplic ...

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MNAzyme qPCR: A Superior Tool for Multiplex qPCR

Multicomponent nucleic acid enzymes (MNAzymes) are nucleic acid enzymes composed of multiple oligonucleotide partzymes that only associate to form catalytic complexes in the presence of a target nucleic acid. Once assembled, MNAzymes cleave a separate substrate (probe) between ...

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Detecting MicroRNA in Human Cancer Tissues with Fluorescence In Situ Hybridization

The technique of nucleic acid in situ hybridization is an effective method for identifying the existence and abundance of nucleic acids in tissue sections or cytological preparations. Such a method has the advantage of keeping morphological relationships intact while identifying ...

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Getting Blood from a Stone: Ultrasensitive Forensic DNA Profiling of Microscopic Bio-Particles Recovered from Touch DNA Evidence

In forensic casework analysis it is sometimes necessary to obtain genetic profiles from increasingly smaller amounts of biological material left behind by persons involved in criminal offenses. The ability to obtain profiles from trace biological evidence is routinely demonst ...

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HAT: A Novel Statistical Approach to Discover Functional Regions in the Genome

Tiling arrays are useful for exploring local functions of regions of the genome in an unbiased fashion. The exact determination of those genomic regions based on tiling-array data, e.g., generated by means of hybridization with immunopreciptated DNA-fragments to the arrays is a challenge. ...

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Integrative Analysis of ChIP-Chip and ChIP-Seq Dataset

Epigenetic regulation and interactions between transcription factors and regulatory genomic regions play crucial roles in controlling transcriptional regulatory networks that drive development, environmental responses, and disease. Chromatin immunoprecipi ...

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Mapping Genomic Features of Tiling Microarray Data by TileMapper

The recent revolution of genomics techniques has allowed the detection of various sequence features and biological variations on whole-genome scale. However, these high-resolution data present significant challenges for experimental biologists to understand and analyz ...

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Evaluation of MeDIP-Chip in the Context of Whole-Genome Bisulfite Sequencing (WGBS-Seq) in Arabidopsis

Studies of DNA methylation in Arabidopsis have rapidly advanced from the analysis of a single reference accession to investigations of large populations. The goal of emerging population studies is to detect differentially methylated regions (DMRs) at the genome-wide scale, and to rel ...

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Application of the Simple and Efficient Mpeak Modeling in Binding Peak Identification in ChIP-Chip Studies

Chromatin immunoprecipitation and hybridization of high-density promoter microarray (ChIP-chip) is a powerful strategy to identify target genes for specific transcription factors and other DNA-binding nuclear proteins in a genome-wide manner. Services of core facilities ...

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Analysis of In Vivo Occupancy of Aebp1, a Transcription Factor, Using High Resolution Tiling Array

Aebp1 is a transcription factor that negatively regulates adipogenesis and also is upregulated in primary GBM as against secondary GBM. Here we describe a systematic approach to map the in vivo occupancy sites of Aebp1 by using a combination of chromatin immune-precipitation technique a ...

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Inference of Alternative Splicing from Tiling Array Data

Alternative splicing (AS) is an important mechanism implicated in eukaryotic gene expression, whereby exon segments of precursor-mRNA transcripts are joined together in different arrangements corresponding to diverse isoforms of mature mRNA. Accumulating evidence sugg ...

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Investigating Gene Promoter Methylation in a Mouse Model of Status Epilepticus

Epigenetic modification of DNA by methylation of the cytosine present in CG dinucleotides constitutes a key regulatory mechanism in the control of gene expression in neurological diseases. In this chapter, we describe an in-depth methodology of methylated DNA immunoprecipitati ...

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